Multi-wavelength immunoassays using surface plasmon-coupled emission

被引：65

作者：

Matveeva, E ^{[1
]}

Malicka, J ^{[1
]}

Gryczynski, I ^{[1
]}

Gryczynski, Z ^{[1
]}

Lakowicz, JR ^{[1
]}

机构：

[1] Univ Maryland, Ctr Fluorescence Spectroscopy, Dept Biochem & Mol Biol, Baltimore, MD 21201 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2004年 / 313卷 / 03期

关键词：

D O I：

10.1016/j.bbrc.2003.12.010

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe a new method for multi-wavelength immunoassays using surface plasmon-coupled emission (SPCE). This phenomenon is coupling of excited fluorophores with a nearby thin metal film, in our case silver, resulting in strongly directional emission into the underlying glass substrate. The angle at which the radiation propagate through the prism depends on the surface plasmon angle for the relevant wavelength. These angles depend on emission wavelength, allowing measurement of multiple analytes using multiple emission wavelengths. We demonstrated this possibility using antibodies labeled with either Rhodamine Red-X or AlexaFluor 647. These antibodies were directed against an antigen protein bound to the silver surface. The emission from each labeled antibody occurred at a different angle on the glass prism, allowing independent measurement of surface binding of each antibody. This method of SPCE immunoassays can be readily extended to 4 or more wavelengths. (C) 2003 Elsevier Inc. All rights reserved.

引用

页码：721 / 726

页数：6

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