Urease inhibitory potential of extracts and active phytochemicals of Hypochaeris radicata (Asteraceae)

被引：15

作者：

Abu-Izneid, Tareq ^{[1
,7
]}

Rauf, Abdur ^{[2
]}

Saleem, Muhammad ^{[3
]}

Mansour, Nashwa ^{[1
,4
]}

Abdelhady, Mohamed I. S. ^{[1
,5
]}

Ibrahim, Munjed M. ^{[1
]}

Patel, Seema ^{[6
]}

机构：

[1] Umm Al Qura Univ, Fac Pharm, Mecca, Saudi Arabia

[2] Univ Swabi, Dept Chem, Khyber Pakhtunkhwa, Pakistan

[3] Univ Educ, Dept Chem, Lahore, Dera Ghazi Khan, Pakistan

[4] Beni Suief Univ, Fac Pharm, Dept Med Chem, Bani Suwayf, Egypt

[5] Helwan Univ, Fac Pharm, Dept Pharmacognosy, Cairo, Egypt

[6] San Diego State Univ, Bioinformat & Med Informat Res Ctr, San Diego, CA 92182 USA

[7] Al Ain Univ Sci & Technol, Coll Pharm, Dept Pharmaceut Sci, Al Ain Campus, Al Ain, U Arab Emirates

来源：

NATURAL PRODUCT RESEARCH | 2020年 / 34卷 / 04期

关键词：

Hypochaeris radicata; phytochemicals; urease inhibition; Carbonic cnhydrase inhibition; CARBONIC-ANHYDRASE; SCOPOLETIN; LEAF;

D O I：

10.1080/14786419.2018.1489387

中图分类号：

O69 [应用化学];

学科分类号：

070301 [无机化学];

摘要：

Urease inhibition potential of compound (1), guaiane-type sesquiterpene (2), confertin (3) and scopoletin (4) was carried out with high throughout mechanism-based assay. These compounds were isolated from Hypochaeris radicata L., an Asteraceae family member. The pure compounds were screened for their urease and carbonic anhydrase inhibitory activities. The ethyl acetate fractions were subjected to column chromatography, which resulted in the isolation and purification of four compounds (1-4). On evaluation, compounds (1-4) exhibited selective activity against urease enzyme with an IC50 value of 180.11 +/- 2.00, 27.18 +/- 0.80, 24.12 +/- 0.2 and 30.12 +/- 1.10 mu M respectively. The compounds (1-4) were found to be inactive against carbonic anhydrase enzyme. Thiourea was used as standard inhibitor (21 +/- 0.14 mu M) of urease enzyme.

引用

页码：553 / 557

页数：5

共 23 条

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