Application of PCR to distinguish common species of dermatophytes

被引:75
作者
Faggi, E
Pini, G
Campisi, E
Bertellini, C
Difonzo, E
Mancianti, F
机构
[1] Univ Florence, Sez Microbiol, Dipartimento Sanita Pubbl, I-50134 Florence, Italy
[2] Univ Florence, Dipartimento Sci Dermatol, I-50121 Florence, Italy
[3] Univ Pisa, Dipartimento Patol Anim, I-56124 Pisa, Italy
关键词
D O I
10.1128/JCM.39.9.3382-3385.2001
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
This report describes the application of PCR fingerprinting for the identification of species and varieties of common dermatophytes and related fungi utilizing as a single primer the simple repetitive oligonucleotide (GACA)(4). The primer was able to amplify all the strains, producing species-specific profiles for Microsporum canis, Microsporum gypseum, Trichophyton rubrum, Trichophyton ajelloi, and Epidermophyton floccosum. Intraspecific variability was not observed for these species. Instead, three different profiles were observed in the Trichophyton mentagrophytes group.
引用
收藏
页码:3382 / 3385
页数:4
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