Diels-Alder ligation and surface immobilization of proteins

被引：126

作者：

de Araújo, AD

Palomo, JM

Cramer, J

Köhn, M

Schröder, H

Wacker, R

Niemeyer, C

Alexandrov, K

Waldmann, H

机构：

[1] Max Planck Inst Mol Physiol, Dept Biol Chem, D-44227 Dortmund, Germany

[2] Univ Dortmund, Fachbereich Chem Biol 3, Dortmund, Germany

[3] Max Planck Inst Mol Physiol, Dept Phys Biochem, D-44227 Dortmund, Germany

[4] Univ Dortmund, Fachbereich Chem Biol 3, Biol Chem Mikrostrukturtech, D-44221 Dortmund, Germany

[5] Chimera Biotec GmbH, D-44227 Dortmund, Germany

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2006年 / 45卷 / 02期

关键词：

Diels-Alder reaction; immobilization; ligation; protein synthesis; proteins;

D O I：

10.1002/anie.200502266

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Figure Presented) Mild attachment: Proteins can be ligated chemoselectively and immobilized on functionalized glass surfaces by means of Diels-Alder cycloadditions (see scheme). © 2006 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：296 / 301

页数：6

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