More than 40,000 transcripts, including novel and noncoding transcripts, in mouse embryonic stem cells

被引：18

作者：

Araki, Ryoko ^{[1
]}

Fukumura, Ryutaro ^{[1
]}

Sasaki, Naokazu ^{[1
]}

Kasama, Yasuji ^{[1
]}

Suzuki, Nobuko ^{[1
]}

Takahashi, Hirokazu ^{[1
]}

Tabata, Yoshimichi ^{[1
]}

Saito, Toshiyuki ^{[1
]}

Abe, Masumi ^{[1
]}

机构：

[1] Natl Inst Radiol Sci, Transcriptome Res Ctr, Inage Ku, Chiba 2638555, Japan

来源：

STEM CELLS | 2006年 / 24卷 / 11期

关键词：

embryonic stem cell; mouse; gene expression profiling; low-abundance transcripts;

D O I：

10.1634/stemcells.2006-0005

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

To study the transcriptome of embryonic stem cells, we used a new gene expression profiling method that can measure the expression levels of unknown and rarely expressed transcripts precisely. We detected a total of 33,136 signal peaks representing transcripts in mouse embryonic stem cells, E14. Subsequent random cloning of the peaks suggests that mouse embryonic stem cells express at least 40,000 transcripts, of which about 2,000 are still unknown. In addition, we identified 1,022 noncoding transcripts, several of which change depending on differentiation in gene expression. Our database provides a high-resolution expression profile of E14 cells and is applicable to other mouse embryonic stem cell analyses. It includes most transcription regulation factor-encoding genes and a significant number of unknown and noncoding transcripts.

引用

页码：2522 / 2528

页数：7

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