Direct observation of cooling of heme upon photodissociation of carbonmonoxy myoglobin

被引：235

作者：

Mizutani, Y ^{[1
]}

Kitagawa, T ^{[1
]}

机构：

[1] OKAZAKI NATL RES INST,INST MOL SCI,OKAZAKI,AICHI 444,JAPAN

来源：

SCIENCE | 1997年 / 278卷 / 5337期

关键词：

D O I：

10.1126/science.278.5337.443

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The formation of vibrationally excited heme upon photodissociation of carbonmonoxy myoglobin and its subsequent vibrational energy relaxation was monitored by picosecond anti-Stokes resonance Raman spectroscopy. The anti-Stokes intensity of the nu(4) band showed immediate generation of vibrationally excited hemes and biphasic decay of the excited populations. The best fit to double exponentials gave time constants of 1.9 +/- 0.6 and 16 +/- 9 picoseconds for vibrational population decay and 3.0 +/- 1.0 and 25 +/- 14 picoseconds for temperature relaxation of the photolyzed heme when a Boltzmann distribution was assumed. The decay of the nu(4) anti-Stokes intensity was accompanied by narrowing and frequency upshift of the Stokes counterpart. This direct monitoring of the cooling dynamics of the heme cofactor within the globin matrix allows the characterization of the vibrational energy flow through the protein moiety and to the water bath.

引用

页码：443 / 446

页数：4

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