Rapid multiplex single nucleotide polymorphism genotyping based on single base extension reactions and color-coded beads

被引：3

作者：

Fujimura, N

Kohara, Y

Okano, K

Yohda, M

Kambara, H

机构：

[1] Tokyo Univ Agr & Technol, Dept Biotechnol & Life Sci, Koganei, Tokyo 1840012, Japan

[2] Hitachi Ltd, Cent Res Lab, Kokubunji, Tokyo 1858601, Japan

来源：

JOURNAL OF BIOSCIENCE AND BIOENGINEERING | 2002年 / 94卷 / 04期

关键词：

SNP genotyping; color-coded beads; single base extension; DNA diagnosis;

D O I：

10.1263/jbb.94.368

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A Single nucleotide polymorphism (SNP) typing method using color-coded beads is promising because it is easy to use and inexpensive. However, the present protocols are not suitable for clinical and diagnostic applications because they need centrifugation for bead-washing. Here, we developed a simplified protocol without a bead-washing procedure that enables SNP typing of PCR amplified fragments in only 30 min.

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收藏

页码：368 / 370

页数：3

相关论文

共 14 条

[1] A microsphere-based assay for multiplexed single nucleotide polymorphism analysis using single base chain extension [J].

Chen, JW ;

Iannone, MA ;

Li, MS ;

Taylor, JD ;

Rivers, P ;

Nelsen, AJ ;

Slentz-Kesler, KA ;

Roses, A ;

Weiner, MP .

GENOME RESEARCH, 2000, 10 (04) :549-557

[2] Parallel genotyping of human SNPs using generic high-density oligonucleotide tag arrays [J].

Fan, JB ;

Chen, XQ ;

Halushka, MK ;

Berno, A ;

Huang, XH ;

Ryder, T ;

Lipshutz, RJ ;

Lockhart, DJ ;

Chakravarti, A .

GENOME RESEARCH, 2000, 10 (06) :853-860

[3]

Iannone MA, 2000, CYTOMETRY, V39, P131, DOI 10.1002/(SICI)1097-0320(20000201)39:2<131::AID-CYTO6>3.0.CO

[4]

2-U

[5] Large-scale discovery and genotyping of single-nucleotide polymorphisms in the mouse [J].

Lindblad-Toh, K ;

Winchester, E ;

Daly, MJ ;

Wang, DG ;

Hirschhorn, JN ;

Laviolette, JP ;

Ardlie, K ;

Reich, DE ;

Robinson, E ;

Sklar, P ;

Shah, N ;

Thomas, D ;

Fan, JB ;

Gingeras, T ;

Warrington, J ;

Patil, N ;

Hudson, TJ ;

Lander, ES .

NATURE GENETICS, 2000, 24 (04) :381-386

[6] Polymorphism identification and quantitative detection of genomic DNA by invasive cleavage of oligonucleotide probes [J].

Lyamichev, V ;

Mast, AL ;

Hall, JG ;

Prudent, JR ;

Kaiser, MW ;

Takova, T ;

Kwiatkowski, RW ;

Sander, TJ ;

de Arruda, M ;

Arco, DA ;

Neri, BP ;

Brow, MAD .

NATURE BIOTECHNOLOGY, 1999, 17 (03) :292-296

[7] Detection of single-base changes using a bioluminometric primer extension assay [J].

Nyren, P ;

Karamohamed, S ;

Ronaghi, M .

ANALYTICAL BIOCHEMISTRY, 1997, 244 (02) :367-373

[8] Full flexibility genotyping of single nucleotide polymorphisms by the GOOD assay [J].

Sauer, Sascha ;

Lechner, Doris ;

Berlin, Kurt ;

Plancon, Christine ;

Heuermann, Arno ;

Lehrach, Hans ;

Gut, Ivo Glynne .

NUCLEIC ACIDS RESEARCH, 2000, 28 (23) :100

[9] Electrophoretic detection of single-nucleotide polymorphisms [J].

See, D ;

Kanazin, V ;

Talbert, H ;

Blake, T .

BIOTECHNIQUES, 2000, 28 (04) :710-+

[10] Multicolor molecular beacons for allele discrimination [J].

Tyagi, S ;

Bratu, DP ;

Kramer, FR .

NATURE BIOTECHNOLOGY, 1998, 16 (01) :49-53