Cloning and expression of the cDNA for canine interleukin-12

被引：17

作者：

Okano, F

Satoh, M

Yamada, K

机构：

[1] Toray Industries, Inc., Chemicals Research Laboratories, Nagoya

[2] Toray Industries, Inc., Minato-ku Nagoya, 455, 9-1, Oe-cho

来源：

JOURNAL OF INTERFERON AND CYTOKINE RESEARCH | 1997年 / 17卷 / 11期

关键词：

D O I：

10.1089/jir.1997.17.713

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We cloned the canine interleukin-12 (IL-12) subunit cDNA. Canine IL-12 exhibited sequence homology to the known sequences of human, mouse, and bovine genes at nucleotide and amino acid levels. Cotransfection of the p35 and p40 subunits of canine IL-12 cDNA clones into COS-1 cells resulted in the secretion of IL-12, which supported proliferation of the stimulated canine lymphocytes, promoted induction of canine interferon-gamma (IFN-gamma) from canine lymphocytes, and showed antitumor effect in vitro. The cloned canine IL-12 will be useful for canine therapeutic applications.

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页码：713 / 718

页数：6

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