Genetic analysis of equine methicillin-resistant Staphylococcus aureus by pulsed-field gel electrophoresis

被引：60

作者：

Shimizu, A ^{[1
]}

Kawano, J ^{[1
]}

Yamamoto, C ^{[1
]}

Kakutani, O ^{[1
]}

Anzai, T ^{[1
]}

Kamada, M ^{[1
]}

机构：

[1] JAPAN RACING ASSOC,EQUINE RES INST,EPIZOOT RES STN,KOKUBUNJI,TOCHIGI,JAPAN

来源：

JOURNAL OF VETERINARY MEDICAL SCIENCE | 1997年 / 59卷 / 10期

关键词：

equine; methicillin-resistant Staphylococcus aureus; pulsed-field gel electrophoresis;

D O I：

10.1292/jvms.59.935

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Pulsed-field gel electrophoresis (PFGE) was used:to determine genetic relationships among 15 methicillin-resistant Staphylococcus aureus (MRSA) isolates from mares with metritis and from a stallion with dermatitis in Hokkaido. All the 15 isolates showed phage pattern 6/47/54/75, coagulase type IV, and enterotoxin type A. The restriction endonuclease SmaI cut their genomic DNAs into 15 or 16 fragments ranging in size from 8 to 630 kb. Fourteen of the 15 isolates showed the same PFGE pattern, whereas the remaining one appeared to be closely related. The 9 human MRSA isolates showing the same phenotypic characteristics as the horse isolates gave different PFGE patterns from those of the horse isolates.

引用

页码：935 / 937

页数：3

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