Quantification and stability of human adenoviruses and polyomavirus JCPyV in wastewater matrices

被引:247
作者
Bofill-Mas, Silvia
Albinana-Gimenez, Nestor
Clemente-Casares, Pilar
Hundesa, Ayalkibet
Rodriguez-Manzano, Jesus
Allard, Annika
Calvo, Miquel
Girones, Rosina
机构
[1] Univ Barcelona, Dept Microbiol, Fac Biol, E-08028 Barcelona, Spain
[2] Univ Barcelona, Dept Stat, Fac Biol, E-08028 Barcelona, Spain
[3] Umea Univ Hosp, Dept Clin Virol, S-90185 Umea, Sweden
关键词
HEPATITIS-E; REAL-TIME; QUANTITATIVE PCR; SEWAGE; POPULATIONS; SHELLFISH; VIRUSES; SPAIN; DNA;
D O I
10.1128/AEM.00965-06
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Human adenoviruses (HAdV) and human polyomavirus JCPyV have been previously proposed as indicators of fecal viral contamination in the environment. Different wastewater matrices have been analyzed by applying real-time quantitative PCR procedures for the presence, quantity, and stability of a wide diversity of excreted HAdV and JCPyV. High quantities of HAdV and JCPyV were detected in sewage, effluent wastewater, sludge, and biosolid samples. Both viruses showed high stability in urban sewage. These results confirm the suitability of both viruses as indicators of human fecal viral pollution.
引用
收藏
页码:7894 / 7896
页数:3
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