Improved PCR method for amplification of GC-Rich DNA sequences

被引：52

作者：

Hubé, F

Reverdiau, P ^{[1
]}

Iochmann, S

Gruel, Y

机构：

[1] INSERM, U618, F-37032 Tours, France

[2] IFR 135, F-37044 Tours, France

来源：

MOLECULAR BIOTECHNOLOGY | 2005年 / 31卷 / 01期

关键词：

GC-rich sequences; CpG island; PCR; promoter region; TFPI-2;

D O I：

10.1385/MB:31:1:081

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Most housekeeping genes, tumor-suppressor genes, and approx 40% of tissue-specific genes contain G+C sequences in their promoter region that were very difficult to amplify. In this report, we propose an improved polymerase chain reaction (PCR) method to be used for successful amplification of the tissue factor pathway inhibitor (TFPI)-2 gene promoter region that exhibit > 70% G+C content in a sequence of approx 300 bp and a complete CpG island region spanning exon 1, the three transcription initiation sites, and the translation start site. Therefore, this method can be recommended to amplify other GC-rich genomic templates.

引用

收藏

页码：81 / 84

页数：4

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