Screening and mutagenesis of molds for improvement of the simultaneous production of catalase and glucose oxidase

被引：55

作者：

Fiedurek, J

Gromada, A

机构：

[1] Dept. of Industrial Microbiology, Maria Curie-Sktodowska University, Lublin

[2] Dept. of Industrial Microbiology, M C Sklowdowska University, 20-033 Lublin

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 1997年 / 20卷 / 05期

关键词：

catalase; glucose oxidase; screening; Aspergillus niger;

D O I：

10.1016/S0141-0229(96)00148-2

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 0836 [生物工程]; 090102 [作物遗传育种]; 100705 [微生物与生化药学];

摘要：

A strain of Aspergillus niger (G-IV-10) most effective for simultaneous production of catalase and glucose oxidase (GOD) was selected out of molds belonging to 15 different species by the method of test-tube microculture. Conidia of the selected strain were further subjected to mutagenesis with both UV and N-methyl-N'-nitro-N-nitrosoguanidine (NTG) and the products were analyzed for catalase activity with our own diffusion plate method. Among 1,055 strains isolated after mutagenesis Eight showed higher catalase (from 44-102.1%) and GOD (from 8.9 to over 77%) activities than the starting Aspergillus niger G-IV-10. (C) 1997 by Elsevier Science Inc.

引用

页码：344 / 347

页数：4

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