Dietary omega-3 polyunsaturated fatty acid does not influence the intestinal microbial communities of broiler chickens

被引:24
作者
Geier, M. S. [1 ,2 ]
Torok, V. A. [2 ,3 ]
Allison, G. E. [4 ,5 ]
Ophel-Keller, K. [2 ,3 ]
Gibson, R. A. [6 ]
Munday, C. [4 ,5 ]
Hughes, R. J. [1 ,2 ]
机构
[1] Univ Adelaide, Pig & Poultry Prod Inst, S Australian Res & Dev Inst, Adelaide, SA 5371, Australia
[2] Univ New England, Australian Poultry Cooperat Res Ctr, Armidale, NSW 2351, Australia
[3] S Australian Res & Dev Inst, Urrbrae, SA 5001, Australia
[4] Australian Natl Univ, Sch Biochem & Mol Biol, Canberra, ACT 0200, Australia
[5] Australian Natl Univ, ANU Med Sch, Canberra, ACT 0200, Australia
[6] Univ Adelaide, Sch Agr Food & Wine, Urrbrae, SA 5064, Australia
关键词
broiler; denaturing gradient gel electrophoresis; polyunsaturated fatty acid; terminal restriction fragment length polymorphism; GRADIENT GEL-ELECTROPHORESIS; BACTERIAL COMMUNITY; N-3; ENRICHMENT; GUT MICROFLORA; FISH-OIL; PERFORMANCE; GROWTH; IDENTIFICATION; LACTOBACILLUS; ALTER;
D O I
10.3382/ps.2009-00126
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
090502 [动物营养与饲料科学];
摘要
The capacity for n-3 polyunsaturated fatty acids (PUFA) to improve broiler chicken growth, influence the intestinal microbial communities, and modify the PUFA content of meat was studied. Male Cobb 500 chickens were fed 1 of 4 diets from hatch: control (standard diet with no additives), ZnB (standard diet with added antibiotics), 2% SALmate (standard diet with 2% SALmate, which is composed of 42% fish oil and 58% starch), and 5% SALmate (standard diet with 5% SALmate). A 7-d energy metabolism study was conducted between d 15 and 22 posthatch. Birds were killed at d 25 and intestinal samples were collected to assess microbial communities by terminal restriction fragment length polymorphism and Lactobacillus PCR-denaturing gradient gel electrophoresis. Diet did not affect BW, feed intake, feed conversion, or ileal digestible energy (P > 0.05). Apparent ME was greater in ZnB-fed birds compared with all other diets (P < 0.05). Breast tissue levels of eicosapentaenoic acid, docosahexaenoic acid, docosapentaenoic acid, and total n-3 PUFA were elevated significantly in 2% SALmate- and 5% SALmate- fed chickens compared with control and ZnB diets (P < 0.05). No significant differences in overall microbial communities were observed in the ileum or cecum as assessed by terminal RFLP (P > 0.05). Birds fed 2% SALmate had a significantly different cecal Lactobacillus species profile compared with birds fed the control diet (P < 0.05); however, no differences were observed in birds fed 5% SALmate compared with birds fed all other diets. In addition to the expected increase in breast tissue n-3 fatty acid levels, a low level of dietary n-3 PUFA also altered the intestinal Lactobacillus species profiles. However, n-3 PUFA supplementation did not alter the overall microbial communities or broiler performance.
引用
收藏
页码:2399 / 2405
页数:7
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