Chondroitin sulphate modification in the α4 chain of human recombinant laminin-8 (α4β1γ1)

被引：22

作者：

Kortesmaa, J

Doi, M

Patarroyo, M

Tryggvason, K ^{[1
]}

机构：

[1] Karolinska Inst, Dept Med Biochem & Biophys, Div Matrix Biol, S-17177 Stockholm, Sweden

[2] BioStratum AB, S-17177 Stockholm, Sweden

[3] Karolinska Inst, Ctr Microbiol & Tumor Biol, S-17177 Stockholm, Sweden

[4] Karolinska Inst, Dept Odontol, S-17177 Stockholm, Sweden

来源：

MATRIX BIOLOGY | 2002年 / 21卷 / 06期

关键词：

laminin-8; recombinant; chondroitin sulphate;

D O I：

10.1016/S0945-053X(02)00052-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have produced human laminin-8 (alpha4beta1gamma1) using recombinant technology. Approximately half of the recombinant laminin-8 (rLN-8) molecules were found to have a chondroitin sulphate modification in the alpha4 chain. The substituted and non-substituted forms were separated and tested for cell adhesion activity. Lower cell adhesion promoting activity was seen for the substituted form, but the integrin receptor utilization was similar. We also found the human rLN-8 to behave identically in cell adhesion assays compared to a human/mouse hybrid variant of rLN-8. (C) 2002 Elsevier Science B.V. and International Society of Matrix Biology. All rights reserved.

引用

页码：483 / 486

页数：4

共 7 条

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[2]

2-R

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