Assay for the determination of urinary 8-hydroxy-2'deoxyguanosine by high-performance liquid chromatography with electrochemical detection

被引：88

作者：

Germadnik, D ^{[1
]}

Pilger, A ^{[1
]}

Rudiger, HW ^{[1
]}

机构：

[1] UNIV VIENNA VIENNA GEN HOSP,DEPT OCCUPAT MED,A-1090 VIENNA,AUSTRIA

来源：

JOURNAL OF CHROMATOGRAPHY B | 1997年 / 689卷 / 02期

关键词：

8-hydroxy-2'-deoxyguanosine; DNA;

D O I：

10.1016/S0378-4347(96)00328-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A high-performance liquid chromatographic procedure with electrochemical detection is described for the determination of urinary 8-hydroxy-2'-deoxyguanosine, a major oxidative DNA lesion induced by radical forming agents. A two-step solid-phase extraction procedure was followed for extracting 8-hydroxy-2'-deoxyguanosine from human urine and the analysis was performed on a RP-18 analytical column under isocratic conditions. The limit of detection of 8-hydroxy-2'-deoxyguanosine in urine was found to be 0.9 nM. The non-invasive assay provides an indirect measurement of oxidative DNA damage.

引用

页码：399 / 403

页数：5

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