Transfer of small YACs to E-coli as large circular plasmids

被引：2

作者：

Frengen, E

Wu, CY

deJong, PJ

机构：

[1] ROSWELL PK CANC INST,DEPT HUMAN GENET,BUFFALO,NY 14263

[2] UNIV OSLO,BIOTECHNOL CTR OSLO,N-0317 OSLO,NORWAY

来源：

GENETIC ANALYSIS-BIOMOLECULAR ENGINEERING | 1997年 / 14卷 / 02期

关键词：

Pl-derived artificial chromosomes; YAC; E-coli;

D O I：

10.1016/S1050-3862(97)00012-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We have designed a YAC circularization vector, pCIRC3, allowing enrichment of the YAC DNA by exonuclease digestion of the linear yeast chromosomes. Due to the presence of P1 replicon sequences in this vector, the circular YACs would replicate as PACs in Escherischia coli. (C) 1997 Elsevier Science B.V.

引用

页码：55 / 59

页数：5

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