A rapid and efficient single-cell manipulation method for screening antigen-specific antibody-secreting cells from human peripheral blood

被引：211

作者：

Jin, Aishun ^{[1
,2
]}

Ozawa, Tatsuhiko ^{[1
]}

Tajiri, Kazuto ^{[1
,3
]}

Obata, Tsutomu ^{[4
]}

Kondo, Sachiko ^{[1
]}

Kinoshita, Koshi ^{[1
]}

Kadowaki, Shinichi ^{[1
]}

Takahashi, Kazuo ^{[5
]}

Sugiyama, Toshiro ^{[3
]}

Kishi, Hiroyuki ^{[1
]}

Muraguchi, Atsushi ^{[1
]}

机构：

[1] Toyama Univ, Dept Immunol, Grad Sch Med & Pharmaceut Sci, Toyama 930, Japan

[2] SC World Inc, Toyama, Japan

[3] Toyama Univ, Dept Internal Med 3, Grad Sch Med & Pharmaceut Sci, Toyama 930, Japan

[4] Toyama Ind Technol Ctr, Cent Res Inst, Toyama 9300866, Japan

[5] Osaka Prefectural Inst Publ Hlth, Dept Infect Dis, Osaka 537, Japan

来源：

NATURE MEDICINE | 2009年 / 15卷 / 09期

关键词：

D O I：

10.1038/nm.1966

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Antigen-specific human monoclonal antibodies (mAbs) are key candidates for therapeutic agents. However, the availability of a suitable screening system for antigen-specific antibody-secreting cells (ASCs) is limited in humans. Here we present a unique method for detecting individual ASCs using microwell array chips, which enables the analysis of live cells on a single-cell basis and offers a rapid, efficient and high-throughput (up to 234,000 individual cells) system for identifying and recovering objective ASCs. We applied the system to detect and retrieve ASCs for hepatitis B virus and influenza viruses from human peripheral blood lymphocytes and produced human mAbs with virus-neutralizing activities within a week. Furthermore, we show that the system is useful for detecting ASCs for multiple antigens as well as for selection of ASCs secreting high-affinity antibodies on a chip. Our method can open the way for the generation of therapeutic antibodies for individual patients.

引用

页码：1088 / U146

页数：6

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