Immunoreactivity of VR1 on epidermal keratinocyte of human skin

被引：192

作者：

Denda, M

Fuziwara, S

Inoue, K

Denda, S

Akamatsu, H

Tomitaka, A

Matsunaga, K

机构：

[1] Shiseido Life Sci Res Ctr, Kanazawa Ku, Yokohama, Kanagawa 2368643, Japan

[2] Fujita Hlth Univ, Sch Med, Dept Dermatol, Toyoake, Aichi 4701192, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2001年 / 285卷 / 05期

关键词：

ion channel; pain sensation; pH; heat; capsaicin;

D O I：

10.1006/bbrc.2001.5299

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We demonstrated the immunoreactivity of the receptor proteins, VR1, ion channels associated with pain sensation, on the epidermis of the human skin. Immunohistochemistry using antiserum against VR1 derived peptide showed immunoreactivity on the keratinocytes cell membrane of the human epidermis and cultured keratinocytes. The blocking peptide of the antiserum reduced the immunoreactivity on the epidermis. RT-PCR assay of cultured human keratinocyte also showed expression of VR1 mRNA. These results suggest the existence of VR1-like protein in epidermal keratinocytes of human skin. (C) 2001 Academic Press.

引用

页码：1250 / 1252

页数：3

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