Oral cancer in vivo gene expression profiling assisted by laser capture microdissection and microarray analysis

被引：194

作者：

Alevizos, I

Mahadevappa, M

Zhang, X

Ohyama, H

Kohno, Y

Posner, M

Gallagher, GT

Varvares, M

Cohen, D

Kim, D

Kent, R

Donoff, RB

Todd, R

Yung, CM

Warrington, JA

Wong, DTW

机构：

[1] Harvard Univ, Sch Dent Med, Dept Oral Med & Diagnost Sci, Div Oral Biol, Boston, MA 02115 USA

[2] Affymetrix Inc, Santa Clara, CA 95051 USA

[3] China Med Univ, Dept Biol, Shenyang 110001, Peoples R China

[4] Dana Farber Canc Inst, Div Hematol Oncol, Dept Med, Boston, MA 02115 USA

[5] Massachusetts Eye & Ear Infirm, Dept Otolaryngol, Boston, MA 02115 USA

[6] Univ Nebraska, Coll Dent, Ctr Med, Lincoln, NE 68583 USA

[7] Forsyth Inst, Boston, MA 02115 USA

[8] Massachusetts Gen Hosp, Dept Oral & Maxillofacial Surg, Boston, MA 02114 USA

[9] Chung Shan Med & Dent Coll, Taichung, Taiwan

来源：

ONCOGENE | 2001年 / 20卷 / 43期

关键词：

oral cancer; gene expression; laser capture microdissection; microarrays;

D O I：

10.1038/sj.onc.1204685

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Large scale gene expression profiling was carried out on laser capture microdissected (LCM) tumor and normal oral epithelial cells and analysed on high-density oligonucleotide microarrays. About 600 genes were found to be oral cancer associated. These oral cancer associated genes include oncogenes, tumor suppressors, transcription factors, xenobiotic enzymes, metastatic proteins, differentiation markers, and genes that have not been implicated in oral cancer. The database created provides a verifiable global profile of gene expression during oral carcinogenesis, revealing the potential role of known genes as well as genes that have not been previously implicated in oral cancer.

引用

页码：6196 / 6204

页数：9

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