Resistance to β-lactam antibiotics in Pseudomonas aeruginosa due to interplay between the MexAB-OprM efflux pump and β-lactamase

被引：62

作者：

Nakae, T ^{[1
]}

Nakajima, A ^{[1
]}

Ono, T ^{[1
]}

Saito, K ^{[1
]}

Yoneyama, H ^{[1
]}

机构：

[1] Tokai Univ, Sch Med, Dept Mol Life Sci, Isehara, Kanagawa 2591193, Japan

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 1999年 / 43卷 / 05期

关键词：

D O I：

10.1128/AAC.43.5.1301

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We evaluated the roles of the MexAB-OprM efflux pump and beta-lactamase in beta-lactam resistance in Pseudomonas aeruginosa by constructing OprM-deficient, OprM basal level, and OprM fully expressed mutants from beta-lactamase-negative, -inducible, and -overexpressed strains. We conclude that, with the notable exception of imipenem, the MexAB-OprM pump contributes significantly to beta-lactam resistance in both beta-lactamase-negative and beta-lactamase-inducible strains, while the contribution of the MexAB-OprM efflux system is negligible in strains with overexpressed beta-lactamase. Overexpression of the efflux pump alone contributes to the high level of beta-lactam resistance in the absence of beta-lactamase.

引用

页码：1301 / 1303

页数：3

共 18 条

[1]

Ausubel FM, 1995, CURRENT PROTOCOLS MO

[2] RESISTANCE OF PSEUDOMONAS-AERUGINOSA MUTANTS WITH ALTERED CONTROL OF CHROMOSOMAL BETA-LACTAMASE TO PIPERACILLIN, CEFTAZIDIME, AND CEFSULODIN [J].

BRYAN, LE ;

KWAN, S ;

GODFREY, AJ .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1984, 25 (03) :382-384

[3]

BRYAN LE, 1979, PSEUDOMONAS AERUGINO, P219

[4] NEW NORFLOXACIN RESISTANCE GENE IN PSEUDOMONAS-AERUGINOSA PAO [J].

FUKUDA, H ;

HOSAKA, M ;

HIRAI, K ;

IYOBE, S .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1990, 34 (09) :1757-1761

[5] MUTATIONS PRODUCING RESISTANCE TO NORFLOXACIN IN PSEUDOMONAS-AERUGINOSA [J].

HIRAI, K ;

SUZUE, S ;

IRIKURA, T ;

IYOBE, S ;

MITSUHASHI, S .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1987, 31 (04) :582-586

[6] Characterization of MexE-MexF-OprN, a positively regulated multidrug efflux system of Pseudomonas aeruginosa [J].

Kohler, T ;

MicheaHamzehpour, M ;

Henze, U ;

Gotoh, N ;

Curty, LK ;

Pechere, JC .

MOLECULAR MICROBIOLOGY, 1997, 23 (02) :345-354

[7] OFLOXACIN-RESISTANT PSEUDOMONAS-AERUGINOSA MUTANTS WITH ELEVATED DRUG EXTRUSION ACROSS THE INNER MEMBRANE [J].

LEI, Y ;

SATO, K ;

NAKAE, T .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1991, 178 (03) :1043-1048

[8] INVALIDITY FOR PSEUDOMONAS-AERUGINOSA OF AN ACCEPTED MODEL OF BACTERIAL PERMEABILITY TO BETA-LACTAM ANTIBIOTICS [J].

LIVERMORE, DM ;

DAVY, KWM .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1991, 35 (05) :916-921

[9] Interplay between chromosomal β-lactamase and the MexAB-OprM efflux system in intrinsic resistance to β-lactams in Pseudomonas aeruginosa [J].

Masuda, N ;

Gotoh, N ;

Ishii, C ;

Sakagawa, E ;

Ohya, S ;

Nishino, T .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1999, 43 (02) :400-402

[10] EXPRESSION OF GENES ASSOCIATED WITH ANTIBIOTIC EXTRUSION IN PSEUDOMONAS-AERUGINOSA [J].

MORSHED, SRM ;

LEI, Y ;

YONEYAMA, H ;

NAKAE, T .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1995, 210 (02) :356-362

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