Silencing of YY1 downregulates RIZ1 promoter in human osteosarcoma

被引：7

作者：

Abbondanza, Ciro ^{[1
]}

de Nigris, Filomena ^{[1
]}

De Rosa, Caterina ^{[1
]}

Rossiello, Raffaele ^{[2
]}

Puca, Giovanni Alfredo ^{[1
]}

Napoli, Claudio ^{[1
]}

机构：

[1] II Univ Naples, Sch Med 1, Dept Gen Pathol, I-80138 Naples, Italy

[2] II Univ Naples, Sch Med 1, Div Human Pathol, I-80138 Naples, Italy

来源：

ONCOLOGY RESEARCH | 2008年 / 17卷 / 01期

关键词：

cancer; RIZ; YY1; ostosarcoma;

D O I：

10.3727/096504008784046081

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 [肿瘤学];

摘要：

RIZ1 isoform, but not RIZ2, is commonly silenced in many types of tumors. In osteosarcoma cells, RIZ1 protein is very abundant. The silencing of YY1 protein, a recent target gene in osteosarcoma cells, reduced the expression of RIZ1 protein. Here we show that RIZ1 overexpression is a transcriptional event documented by Western blot, RT-PCR, and promoter assays. YY1 protein binds and cooperates to positive regulation of the RIZ1 promoter and its presence reduced the dimethyl lysine 9 histone 3 by chromatin immunoprecipitation assays. These results indicate that overexpression of YY1 in osteosarcoma cells plays a key role in positive regulation of RIZ1. The coexpression of RIZ1/YY1 proteins suggests a tandem regulatory mechanism in human osteosarcoma cells and tissues.

引用

页码：33 / 41

页数：9

共 35 条

[1]

The retinoblastoma-interacting zinc-finger protein RIZ is a downstream effector of estrogen action [J].

Abbondanza, C ;

Medici, N ;

Nigro, V ;

Rossi, V ;

Gallo, L ;

Piluso, G ;

Belsito, A ;

Roscigno, A ;

Bontempo, P ;

Puca, AA ;

Molinari, AM ;

Moncharmont, B ;

Puca, GA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (07) :3130-3135

[2]

Yin Yang 1 cooperates with activator protein 2 to stimulate ERBB2 gene expression in mammary cancer cells [J].

Begon, DY ;

Delacroix, L ;

Vernimmen, D ;

Jackers, P ;

Winkler, R .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2005, 280 (26) :24428-24434

[3]

A histone methyltransferase is required for maximal response to female sex hormones [J].

Carling, T ;

Kim, KC ;

Yang, XH ;

Gu, H ;

Zhang, XK ;

Huang, S .

MOLECULAR AND CELLULAR BIOLOGY, 2004, 24 (16) :7032-7042

[4]

Candidate tumor suppressor RIZ is frequently involved in colorectal carcinogenesis [J].

Chadwick, RB ;

Jiang, CL ;

Bennington, GA ;

Yuan, B ;

Johnson, CK ;

Stevens, MW ;

Niemann, TH ;

Peltomaki, P ;

Huang, S ;

de la Chapelle, A .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (06) :2662-2667

[5]

Cooperation between Myc and YY1 provides novel silencing transcriptional targets of α3β1-integrin in tumour cells [J].

de Nigris, F. ;

Botti, C. ;

Rossiello, R. ;

Crimi, E. ;

Sica, V. ;

Napoli, C. .

ONCOGENE, 2007, 26 (03) :382-394

[6]

Expression of transcription factor Yin Yang 1 in human osteosarcomas [J].

de Nigris, Filomena ;

Botti, Chiara ;

de Chiara, Anna ;

Rossiello, Raffaele ;

Apice, Gaetano ;

Fazioli, Flavio ;

Fiorito, Carmela ;

Sica, Vincenzo ;

Napoli, Claudio .

EUROPEAN JOURNAL OF CANCER, 2006, 42 (15) :2420-2424

[7]

Du Y, 2001, CANCER RES, V61, P8094

[8]

Clinicopathological significance of microsatellite instability and mutated RIZ in colorectal cancer [J].

Emterling, A ;

Wallin, Å ;

Arbman, G ;

Sun, XF .

ANNALS OF ONCOLOGY, 2004, 15 (02) :242-246

[9]

Preferential loss of a polymorphic RIZ allele in human hepatocellular carcinoma [J].

Fang, W ;

Piao, Z ;

Buyse, IM ;

Simon, D ;

Sheu, JC ;

Perucho, M ;

Huang, S .

BRITISH JOURNAL OF CANCER, 2001, 84 (06) :743-747

[10]

Histone methylation-dependent mechanisms impose ligand dependency for gene activation by nuclear receptors [J].

Garcia-Bassets, Ivan ;

Kwon, Young-Soo ;

Telese, Francesca ;

Prefontaine, Gratien G. ;

Hutt, Kasey R. ;

Cheng, Christine S. ;

Ju, Bong-Gun ;

Ohgi, Kenneth A. ;

Wang, Jianxun ;

Escoubet-Lozach, Laure ;

Rose, David W. ;

Glass, Christopher K. ;

Fu, Xiang-Dong ;

Rosenfeld, Michael G. .

CELL, 2007, 128 (03) :505-518

← 1 2 3 4 →