Bovine anaplasmosis prevalence in northern Veracruz state, Mexico

被引:19
作者
CossioBayugar, R [1 ]
Rodriguez, SD [1 ]
GarciaOrtiz, MA [1 ]
GarciaTapia, D [1 ]
AboytesTorres, R [1 ]
机构
[1] CTR NACL INVEST DISCIPLINARIAS PARASITOL VET,JIUTEPEC 62550,MORELOS,MEXICO
关键词
cattle; PCR; complement fixation; Anaplasma marginale;
D O I
10.1016/S0167-5877(97)00016-0
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
In order to learn more about the presence of bovine anaplasmosis in northern Veracruz sate, Mexico, paired blood and serum samples from 368 cattle were subjected to polymerase chain reaction (PCR) and complement-fixation test (CFt). The overall prevalence of Anaplasma marginale by PCR was 69.2% and seroprevalence by CFt 54.6%. Age-specific prevalence was calculated for each test. Sixty-eight percent of animals from 0 to 3 months of age already were infected (PCR-positive), compared to only 42.4% positive by serology. CFt results suggested that presence of antibody increases with age up to 18 to 36 months, decreasing thereafter. Presence of the rickettsia seems to follow the same early pattern but with a new increase in animals 36 months or older. Serology results provided a biased picture of the true prevalence of anaplasmosis. Calculated specificity and sensitivity (63.5% and 68.2%) for CFt using PCR values as true values, appear very low and unreliable. The data generated by DNA-based surveys seem more appropriate to help design and implement control or eradication programs for bovine anaplasmosis. (C) 1997 Elsevier Science B.V.
引用
收藏
页码:165 / 170
页数:6
相关论文
共 22 条
[1]   DEVELOPMENT OF A RECOMBINANT ANAPLASMA-MARGINALE DNA PROBE [J].
ABOYTESTORRES, R ;
BUENING, GM .
VETERINARY MICROBIOLOGY, 1990, 24 (3-4) :391-408
[2]  
ABOYTESTORRES R, 1992, THESIS U MISSOURI CO
[3]  
AMBROSIO RE, 1990, REV SCI OFF INT EPIZ, V9, P795
[4]  
[Anonymous], 1990, PCR PROTOCOLS GUIDE
[5]   SIMPLE AND EFFICIENT SYSTEM FOR SYNTHESIS OF NONRADIOACTIVE NUCLEIC-ACID HYBRIDIZATION PROBES USING PCR [J].
EMANUEL, JR .
NUCLEIC ACIDS RESEARCH, 1991, 19 (10) :2790-2790
[6]   REDUCTION OF BACKGROUND PROBLEMS IN NONRADIOACTIVE NORTHERN AND SOUTHERN BLOT ANALYSES ENABLES HIGHER SENSITIVITY THAN P-32 BASED HYBRIDIZATIONS [J].
ENGLERBLUM, G ;
MEIER, M ;
FRANK, J ;
MULLER, GA .
ANALYTICAL BIOCHEMISTRY, 1993, 210 (02) :235-244
[7]   AN APPLICATION OF SAMPLING THEORY IN ANIMAL-DISEASE PREVALENCE SURVEY DESIGN [J].
FARVER, TB ;
THOMAS, C ;
EDSON, RK .
PREVENTIVE VETERINARY MEDICINE, 1985, 3 (05) :463-473
[8]  
Figueroa J V, 1993, Rev Elev Med Vet Pays Trop, V46, P71
[9]   MULTIPLEX POLYMERASE CHAIN-REACTION BASED ASSAY FOR THE DETECTION OF BABESIA-BIGEMINA, BABESIA-BOVIS AND ANAPLASMA-MARGINALE DNA IN BOVINE BLOOD [J].
FIGUEROA, JV ;
CHIEVES, LP ;
JOHNSON, GS ;
BUENING, GM .
VETERINARY PARASITOLOGY, 1993, 50 (1-2) :69-81
[10]  
GARCIA E, 1987, MODIFICACIONES AL SI, P72