Primer/template-independent synthesis of poly d(A-T) by Taq polymerase

被引：28

作者：

Hanaki, K

Odawara, T

Muramatsu, T

Kuchino, Y

Masuda, M

Yamamoto, K

Nozaki, C

Mizuno, K

Yoshikura, H

机构：

[1] UNIV TOKYO, FAC MED, DEPT BACTERIOL, BUNKYO KU, TOKYO 113, JAPAN

[2] NATL CANC CTR, RES INST, DIV BIOPHYS, CHUO KU, TOKYO 104, JAPAN

[3] UNIV TOKYO, BUNIN HOSP, LAB CLIN MICROBIOL & IMMUNOL, BUNKYO KU, TOKYO 113, JAPAN

[4] CHEMOSEROTHERAPEUT RES INST, KYOKUSHI, KUMAMOTO 86912, JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1997年 / 238卷 / 01期

关键词：

D O I：

10.1006/bbrc.1997.7197

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Tag DNA polymerase polymerized dATP and dTTP to poly d(A-T) without requiring added primer/template in the temperature range of 60-70 degrees C, Tth DNA polymerase also catalyzed the reaction, while Delta Tth, Vent, Vent(exo-), Pfu, Ultma, BcaBEST, and KOD DNA polymerases did not, The reaction was distinct from the template-nonrequiring terminal deoxynucleotidyl transferase reaction which absolutely required primers. (C) 1997 Academic Press.

引用

页码：113 / 118

页数：6

共 28 条

[1]

Arakawa T, 1996, DNA Res, V3, P87, DOI 10.1093/dnares/3.2.87

[2]

BOLLUM JF, 1974, ENZYMES, V10, P145

[3]

EFFECT OF INCUBATION CONDITIONS ON NUCLEOTIDE SEQUENCE OF DNA PRODUCTS OF UNPRIMED DNA POLYMERASE REACTIONS [J].