Methods for the determination of plasma total homocysteine:: a review

被引：29

作者：

Amores-Sánchez, MI

Medina, MA ^{[1
]}

机构：

[1] Univ Malaga, Fac Sci, Dept Mol Biol & Biochem, E-29071 Malaga, Spain

[2] Cty Hosp Axarquia, Malaga, Spain

来源：

CLINICAL CHEMISTRY AND LABORATORY MEDICINE | 2000年 / 38卷 / 03期

关键词：

homocysteine; reduction procedure; derivatization; HPLC; mass spectrometry; enzyme immunoassay;

D O I：

10.1515/CCLM.2000.028

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

Total homocysteine is the sum of all homocysteine species present in plasma. This parameter is valuable for the diagnosis and follow up of cobalamine or folate deficiencies, and it has been accepted as an independent risk factor for premature cardiovascular disease. These reasons justify the introduction of total homocysteine assays in the routine clinical laboratory. In the present review, we discuss the procedures for sample collection, processing and storage, and review the methodologies for measuring total homocysteine. We compare their feasibility as routine methods in the clinical laboratory.

引用

页码：199 / 204

页数：6

共 48 条

[1] Stabilization of blood homocysteine by 3-deazaadenosine [J].