Unsaturated fatty acids bind Myc-Max transcription factor and inhibit Myc-Max-DNA complex formation

被引：18

作者：

Chung, S ^{[1
]}

Park, S ^{[1
]}

Yang, CH ^{[1
]}

机构：

[1] Seoul Natl Univ, Sch Chem & Mol Engn, Seoul 151742, South Korea

来源：

CANCER LETTERS | 2002年 / 188卷 / 1-2期

关键词：

Myc; Max; Mad; heterodimer; E-box; unsaturated fatty acids; SNU16; cells; gene regulation; tumorigenesis; gene expression; linoleic acid; proliferation;

D O I：

10.1016/S0304-3835(02)00455-X

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Oncoprotein Myc, hetero-dimerized with Max through a b/HLH/Zip region, is a transcription factor that governs important cellular processes such as cell cycle entry, proliferation and differentiation. We found that linoleic acid, isolated from Pollen Typhae, and other unsaturated fatty acids have strong inhibitory effects on the binding of Myc-Max heterodimer to an E-box DNA site (CA(C/T)GTG). The interaction of a fatty acid with a protein dimer, not with DNA, is assumed to block the entire Myc-Max-DNA complex formation. Unsaturated fatty acids also showed cytotoxicity against a SNU16 human stomach cancer cell line and conjugated linoleic acid suppressed mRNA expression of several myc-target genes; ornithine decarboxylase, p53, cdc25a in the SNU16 cells. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：153 / 162

页数：10

共 47 条

[1] Mechanism of apoptosis in HL-60 cells induced by n-3 and n-6 polyunsaturated fatty acids [J].

Arita, K ;

Kobuchi, H ;

Utsumi, T ;

Takehara, Y ;

Akiyama, J ;

Horton, AA ;

Utsumi, K .

BIOCHEMICAL PHARMACOLOGY, 2001, 62 (07) :821-828

[2] Modulation of antioxidant enzymes and apoptosis in mice by dietary lipids and treadmill exercise [J].

Avula, CPR ;

Fernandes, G .

JOURNAL OF CLINICAL IMMUNOLOGY, 1999, 19 (01) :35-44

[3] MAD - A HETERODIMERIC PARTNER FOR MAX THAT ANTAGONIZES MYC TRANSCRIPTIONAL ACTIVITY [J].

AYER, DE ;

KRETZNER, L ;

EISENMAN, RN .

CELL, 1993, 72 (02) :211-222

[4] Decrease in linoleic acid metabolites as a potential mechanism in cancer risk reduction by conjugated linoleic acid [J].

Banni, S ;

Angioni, E ;

Casu, V ;

Melis, MP ;

Carta, G ;

Corongiu, FP ;

Thompson, H ;

Ip, C .

CARCINOGENESIS, 1999, 20 (06) :1019-1024

[5]

Bello-Fernandez C, 1992, Curr Top Microbiol Immunol, V182, P445

[6] ONCOGENES IN RETROVIRUSES AND CELLS - BIOCHEMISTRY AND MOLECULAR-GENETICS [J].

BISTER, K ;

JANSEN, HW .

ADVANCES IN CANCER RESEARCH, 1986, 47 :99-188

[7] MAX - A HELIX-LOOP-HELIX ZIPPER PROTEIN THAT FORMS A SEQUENCE-SPECIFIC DNA-BINDING COMPLEX WITH MYC [J].

BLACKWOOD, EM ;

EISENMAN, RN .

SCIENCE, 1991, 251 (4998) :1211-1217

[8]

Boudreau MD, 2001, CANCER RES, V61, P1386

[9]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[10]

CLAKE SD, 1994, ANNU REV NUTR, V14, P83

← 1 2 3 4 5 →