Isolation of a gene from Burkholderia cepacia IS-16 encoding a protein that facilitates phosphatase activity

被引:37
作者
Rodríguez, H
Rossolini, GM
Gonzalez, T
Li, JP
Glick, BR [1 ]
机构
[1] Univ Waterloo, Dept Biol, Waterloo, ON N2L 3G1, Canada
[2] Cuban Res Inst Sugarcane By Prod, Dept Microbiol, Havana, Cuba
[3] Univ Siena, Dept Mol Biol, I-53100 Siena, Italy
关键词
D O I
10.1007/s002840010071
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A genomic library from Burkholderia cepacia IS-16 was constructed in Escherichia coli by partial Sau3AI digestion of the chromosomal DNA, with the plasmid vector Bluescript SK. This library was screened for clones able to grow as green stained colonies on selective medium developed for detecting phosphatase-positive colonies. Three green-stained clones (pFS1, pFS2, and pFS3) carried recombinant plasmids harboring DNA inserts of 5.0, 8.0, and 0.9 kb, respectively, DNA hybridization experiments demonstrated the presence of overlapping DNA fragments in the three clones and that these three clones were all derived from Burkholderia in cepacia IS-16 genomic DNA. DNA sequence analysis, together with polyacrylamide gels of proteins encoded by E. coli containing pFS3, suggested that the isolated 0.9-kb DNA fragment encodes the functional portion of a phosphate transport protein.
引用
收藏
页码:362 / 366
页数:5
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