Meta-topolin, a highly active aromatic cytokinin from poplar leaves (Populus x canadensis Moench, cv Robusta)

被引：103

作者：

Strnad, M

Hanus, J

Vanek, T

Kaminek, M

Ballantine, JA

Fussell, B

Hanke, DE

机构：

[1] ASCR,INST EXPT BOT,OLOMOUC 78371,CZECH REPUBLIC

[2] INST EXPT BOT,ISOTOPE LAB,PRAGUE 14220 4,CZECH REPUBLIC

[3] ACAD SCI CZECH REPUBL,INST ORGAN CHEM & BIOCHEM,PRAGUE 16000 6,CZECH REPUBLIC

[4] INST EXPT BOT,DEPT PLANT GROWTH & DEV,PRAGUE 16630 6,CZECH REPUBLIC

[5] UNIV COLL SWANSEA,DEPT CHEM,SWANSEA SA2 8PP,W GLAM,WALES

[6] UNIV CAMBRIDGE,DEPT PLANT SCI,CAMBRIDGE CB2 3EA,ENGLAND

来源：

PHYTOCHEMISTRY | 1997年 / 45卷 / 02期

关键词：

Populus x canadensis Moench; cv Robusta; Salicaceae; aromatic cytokinin; N-6-(meta-hydroxybenzyl)adenine; meta-topolin;

D O I：

10.1016/S0031-9422(96)00816-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A rapid analytical screening by high-performance liquid chromatography combined with enzymelinked immunosorbent assay specific for N-6-(meta-hydroxybenzyl)adenine and its 9-substituted derivatives revealed several regions of immunoactivity in extracts of mature poplar leaves (Populusxcanadensis Moench., cv. Robusta). The identity of the cross-reactive compounds was investigated by UV and mass spectrometry and after permethylation of unknown substances by gas chromatography-mass spectrometry. Physico-chemical analysis indicated the putative cytokinins are N-6-(meta-hydroxybenzyl)adenine and its 9-beta-D-ribofuranosyl or 9-beta-D-glucopyranosyl derivatives. The chemical structure was subsequently confirmed by synthesis. For N-6(meta-hydroxybenzyl)adenine, a highly active aromatic cytokinin, we propose the trivial name 'meta-topolin'. (C) 1997 Elsevier Science Ltd.

引用

页码：213 / 218

页数：6

共 28 条

[1]

[Anonymous], 1992, BIOL PLANTARUM

[2]

BUDAVARI S, 1989, MERCK INDEX, P1209

[3]

DASNEVES HJC, 1980, BIOCHEM BIOPH RES CO, V95, P1387

[4] ISOLATION AND IDENTIFICATION OF A NEW, NATURALLY-OCCURRING CYTOKININ (6-BENZYLAMINOPURINERIBOSIDE) FROM AN ANISE CELL-CULTURE (PIMPINELLA-ANISUM L) [J].

ERNST, D ;

SCHAFER, W ;

OESTERHELT, D .

PLANTA, 1983, 159 (03) :222-225

[5]

HEWETT EW, 1973, PLANTA, V114, P119, DOI 10.1007/BF00387470

[6]

HOLY FW, 1944, J AM CHEM SOC, V66, P1875

[7]

HORGAN R, 1992, PHYSIOLOGY AND BIOCHEMISTRY OF CYTOKININS IN PLANTS, P3

[8] NEW CYTOKININ FROM POPULUS X ROBUSTA [J].

HORGAN, R ;

HEWETT, EW ;

HORGAN, JM ;

PURSE, J ;

WAREING, PF .

PHYTOCHEMISTRY, 1975, 14 (04) :1005-1008

[9]

HORGAN R, 1973, Tetrahedron Letters, V30, P2827

[10] QUANTITATIVE STRUCTURE-ACTIVITY RELATIONSHIP OF CYTOKININ-ACTIVE ADENINE AND UREA DERIVATIVES [J].

IWAMURA, H ;

FUJITA, T ;

KOYAMA, S ;

KOSHIMIZU, K ;

KUMAZAWA, Z .

PHYTOCHEMISTRY, 1980, 19 (07) :1309-1319

← 1 2 3 →