Prospective identification, isolation, and systemic transplantation of multipotent mesenchymal stem cells in murine bone marrow

被引：621

作者：

Morikawa, Satoru ^{[1
,2
]}

Mabuchi, Yo ^{[1
]}

Kubota, Yoshiaki ^{[3
]}

Nagai, Yasuo ^{[1
]}

Niibe, Kunimichi ^{[1
,2
]}

Hiratsu, Emi ^{[1
]}

Suzuki, Sadafumi ^{[1
]}

Miyauchi-Hara, Chikako ^{[1
,6
]}

Nagoshi, Narihito ^{[1
,4
]}

Sunabori, Takehiko ^{[1
]}

Shimmura, Shigeto ^{[5
]}

Miyawaki, Atsushi ^{[6
,7
]}

Nakagawa, Taneaki ^{[2
]}

Suda, Toshio ^{[3
]}

Okano, Hideyuki ^{[1
]}

Matsuzaki, Yumi ^{[1
]}

机构：

[1] Keio Univ, Dept Physiol, Sch Med, Shinjuku Ku, Tokyo 1608582, Japan

[2] Keio Univ, Dept Dent & Oral Surg, Sch Med, Shinjuku Ku, Tokyo 1608582, Japan

[3] Keio Univ, Dept Cell Differentiat, Sch Med, Sakaguchi Lab Dev Biol,Shinjuku Ku, Tokyo 1608582, Japan

[4] Keio Univ, Dept Orthoped Surg, Sch Med, Shinjuku Ku, Tokyo 1608582, Japan

[5] Keio Univ, Dept Ophthalmol, Sch Med, Shinjuku Ku, Tokyo 1608582, Japan

[6] Inst Phys & Chem Res, Lab Cell Funct & Dynam, Adv Technol Dev Grp, Brain Sci Inst, Wako, Saitama 3510198, Japan

[7] Japan Sci & Technol Agcy, Life Funct & Dynam Exploratory Res Adv Technol Of, Wako, Saitama 3510198, Japan

来源：

JOURNAL OF EXPERIMENTAL MEDICINE | 2009年 / 206卷 / 11期

关键词：

STROMAL CELLS; IN-VIVO; HEMATOPOIETIC MICROENVIRONMENT; NONHEMATOPOIETIC TISSUES; FUNCTIONAL-PROPERTIES; PROGENITOR CELLS; SIDE POPULATION; ADULT MARROW; INBRED MICE; HOST ORIGIN;

D O I：

10.1084/jem.20091046

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Mesenchymal stem cells (MSCs) are defined as cells that undergo sustained in vitro growth and can give rise to multiple mesenchymal lineages. Because MSCs have only been isolated from tissue in culture, the equivalent cells have not been identified in vivo and little is known about their physiological roles or even their exact tissue location. In this study, we used phenotypic, morphological, and functional criteria to identify and prospectively isolate a subset of MSCs (PDGFR alpha(+)Sca-1(+)CD45(-)TER119(-)) from adult mouse bone marrow. Individual MSCs generated colonies at a high frequency and could differentiate into hematopoietic niche cells, osteoblasts, and adipocytes after in vivo transplantation. Naive MSCs resided in the perivascular region in a quiescent state. This study provides the useful method needed to identify MSCs as defined in vivo entities.

引用

页码：2483 / 2496

页数：14

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