DNA polyplexes based on degradable oligoethylenimine-derivatives: Combination with EGF receptor targeting and endosomal release functions

被引：33

作者：

Kloeckner, Julia

Boeckle, Sabine

Persson, Daniel

Roedl, Wolfgang

Ogris, Manfred

Berg, Kristian

Wagner, Ernst

机构：

[1] Univ Munich, Dept Pharm, D-81377 Munich, Germany

[2] Norwegian Radium Hosp, Dept Radiat Biol, Inst Canc Res, N-0310 Oslo, Norway

来源：

JOURNAL OF CONTROLLED RELEASE | 2006年 / 116卷 / 02期

关键词：

endosomal release; epidermal growth factor; melittin; nonviral vectors; oligoethylenimine; photochemical internalization; polyplex;

D O I：

10.1016/j.jconrel.2006.07.002

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Combination of the degradable polymeric gene carriers OEI-HD-1 and LT-OEI-HD-1 with an EGF targeting conjugate resulted in strongly (up to 900-fold) enhanced polyplex activity in EGF-receptor rich HUH7 hepatocellular carcinoma cells. The targeting ligand effect was DNA dose dependent, could be blocked by competitive receptor binding with unbound EGF ligand, and was not observed in receptor-negative control cells. Measures which enhance intracellular endosomal escape, either photochemically enhanced intracellular release (PCI) or the incorporation of a novel membrane-active melittin analog NMA-3, further enhanced gene transfer activity of EGF/OEI-HD-1 polyplexes. (c) 2006 Elsevier B.V. All rights reserved.

引用

收藏

页码：115 / 122

页数：8

相关论文

共 39 条

[1] Structure/property studies of polymeric gene delivery using a library of poly(β-amino esters) [J].

Anderson, DG ;

Akinc, A ;

Hossain, N ;

Langer, R .

MOLECULAR THERAPY, 2005, 11 (03) :426-434

[2]

Berg K, 1999, CANCER RES, V59, P1180

[3] C- versus N-terminally linked metittin-polyethylenimine conjugates: the site of linkage strongly influences activity of DNA potyplexes [J].

Boeckle, S ;

Wagner, E ;

Ogris, M .

JOURNAL OF GENE MEDICINE, 2005, 7 (10) :1335-1347

[4] Melittin analogs with high lytic activity at endosomal pH enhance transfection with purified targeted PEI polyplexes [J].

Boeckle, Sabine ;

Fahrmeir, Julia ;

Roedl, Wolfgang ;

Ogris, Manfred ;

Wagner, Ernst .

JOURNAL OF CONTROLLED RELEASE, 2006, 112 (02) :240-248

[5] Synthesis of linear polyethylenimine derivatives for DNA transfection [J].

Brissault, B ;

Kichler, A ;

Guis, C ;

Leborgne, C ;

Danos, O ;

Cheradame, H .

BIOCONJUGATE CHEMISTRY, 2003, 14 (03) :581-587

[6] Gene delivery with synthetic (non viral) carriers [J].

Brown, MD ;

Schätzlein, AG ;

Uchegbu, IF .

INTERNATIONAL JOURNAL OF PHARMACEUTICS, 2001, 229 (1-2) :1-21

[7] Side-effects of a systemic injection of linear polyethylenimine-DNA complexes [J].

Chollet, P ;

Favrot, MC ;

Hurbin, A ;

Coll, JL .

JOURNAL OF GENE MEDICINE, 2002, 4 (01) :84-91

[8] TRANSFERRIN POLYCATION-MEDIATED INTRODUCTION OF DNA INTO HUMAN LEUKEMIC-CELLS - STIMULATION BY AGENTS THAT AFFECT THE SURVIVAL OF TRANSFECTED DNA OR MODULATE TRANSFERRIN RECEPTOR LEVELS [J].

COTTEN, M ;

LANGLEROUAULT, F ;

KIRLAPPOS, H ;

WAGNER, E ;

MECHTLER, K ;

ZENKE, M ;

BEUG, H ;

BIRNSTIEL, ML .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (11) :4033-4037

[9] Cationic polymer based gene delivery systems [J].

De Smedt, SC ;

Demeester, J ;

Hennink, WE .

PHARMACEUTICAL RESEARCH, 2000, 17 (02) :113-126

[10] PCI-enhanced adenoviral transduction employs the known uptake mechanism of adenoviral particles [J].

Engesæter, BO ;

Bonsted, A ;

Berg, K ;

Hogset, A ;

Engebråten, O ;

Fodstad, O ;

Curiel, DT ;

Mælandsmo, GM .

CANCER GENE THERAPY, 2005, 12 (05) :439-448

← 1 2 3 4 →