Precision-cut slice culture method for rat placenta

被引：10

作者：

Gilligan, J. ^{[2
]}

Tong, M. ^{[2
]}

Longato, L. ^{[2
]}

de la Monte, S. M. ^{[2
,3
,4
]}

Gundogan, F. ^{[1
,4
]}

机构：

[1] Brown Univ, Women & Infants Hosp, Providence, RI 02905 USA

[2] Rhode Isl Hosp, Dept Med, Liver Res Ctr, Providence, RI 02903 USA

[3] Rhode Isl Hosp, Dept Pathol, Providence, RI 02902 USA

[4] Brown Univ, Warren Alpert Med Sch, Providence, RI 02905 USA

来源：

PLACENTA | 2012年 / 33卷 / 01期

关键词：

Placenta; Slice culture; Ethanol; Methodology; ASPARAGINYL BETA-HYDROXYLASE; GROWTH-FACTOR; MITOCHONDRIAL DYSFUNCTION; HEPATOCELLULAR-CARCINOMA; OXIDATIVE STRESS; PREGNANCY LOSS; INSULIN; OVEREXPRESSION; INHIBITION; METABOLISM;

D O I：

10.1016/j.placenta.2011.10.013

中图分类号：

Q [生物科学];

学科分类号：

090105 [作物生产系统与生态工程];

摘要：

Primary trophoblasts, placental explants, and cell line cultures are commonly used to investigate placental development, physiology, and pathology, particularly in relation to pregnancy outcomes. Organotypic slice cultures are increasingly used in other systems because they maintain the normal three-dimensional tissue architecture and have all cell types represented. Herein, we demonstrate the utility of the precision-cut placental slice culture model for studying trophoblastic diseases. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：67 / 72

页数：6

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