Genetic and physical mapping of homologues of the virus resistance gene Rx1 and the cyst nematode resistance gene Gpa2 in potato

被引：25

作者：

Bakker, E. ^{[1
]}

Butterbach, P. ^{[1
]}

van der Voort, J. Rouppe ^{[1
]}

van der Vossen, E. ^{[1
]}

van Vliet, J. ^{[1
]}

Bakker, J. ^{[1
]}

Goverse, A. ^{[1
]}

机构：

[1] Univ Wageningen & Res Ctr, Grad Sch Expt Plant Sci, Dept Nematol, NL-6700 ES Wageningen, Netherlands

来源：

THEORETICAL AND APPLIED GENETICS | 2003年 / 106卷 / 08期

关键词：

Globodera pallida; Potato cyst nematodes; Potato virus X; Resistance gene homologues;

D O I：

10.1007/s00122-003-1213-4

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Nine resistance gene homologues (RGHs) were identified in two diploid potato clones (SH and RH), with a specific primer pair based on conserved motifs in the LRR domain of the potato cyst nematode resistance gene Gpa2 and the potato virus X resistance gene Rx1. A modified AFLP method was used to facilitate the genetic mapping of the RGHs in the four haplotypes under investigation. All nine RGHs appeared to be located in the Gpa2/Rx1 cluster on chromosome XII. Construction of a physical map using bacterial artificial chromosome (BAC) clones for both the Solanum tuberosum ssp. tuberosum and the S. tuberosum ssp. andigena haplotype of SH showed that the RGHs are located within a stretch of less than 200 kb. Sequence analysis of the RGHs revealed that they are highly similar (93 to 95%) to Gpa2 and Rx1. The sequence identities among all RGHs range from 85 to 100%. Two pairs of RGHs are identical, or nearly so (100 and 99.9%), with each member located in a different genotype. Southern-blot analysis on genomic DNA revealed no evidence for additional homologues outside the Gpa2/Rx1 cluster on chromosome XII.

引用

页码：1524 / 1531

页数：8

共 23 条

[1] The R1 gene for potato resistance to late blight (Phytophthora infestans) belongs to the leucine zipper/NBS/LRR class of plant resistance genes [J].