DNA end-joining catalyzed by human cell-free extracts

被引：273

作者：

Baumann, P ^{[1
]}

West, SC ^{[1
]}

机构：

[1] Imperial Canc Res Fund, Clare Hall Labs, S Mimms EN6 3LD, Herts, England

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1998年 / 95卷 / 24期

关键词：

DNA repair; recombination; Ku protein; DNA-PK; DNA ligase;

D O I：

10.1073/pnas.95.24.14066

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 [理学]; 0710 [生物学]; 09 [农学];

摘要：

Mammalian cells defective in DNA end-joining are highly sensitive to ionizing radiation and are immunodeficient because of a failure to complete V(D)J recombination. By using cell-free extracts prepared from human lymphoblastoid cell lines, an in vitro system for end-joining has been developed. Intermolecular ligation was found to be accurate and to depend on DNA ligase IV/Xrcc4 and requires Ku70, Ku86, and DNA-PKcs, the three subunits of the DNA-activated protein kinase DNA-PK. Because these activities are involved in the cellular resistance to x-irradiation and V(D)J recombination, the development of this in vitro system provides an important advance in the study of the mechanism of DNA end-joining in human cells.

引用

页码：14066 / 14070

页数：5

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