A molecular-capsular-type prediction system for 90 Streptococcus pneumoniae serotypes using partial cpsA-cpsB sequencing and wzy- or wzx-specific PCR

被引:51
作者
Kong, FR
Wang, WZ
Tao, JN
Wang, L
Wang, Q
Sabananthan, A
Gilbert, GL [1 ]
机构
[1] ICPMR, CIDM, Westmead, NSW 2145, Australia
[2] Wuhan First Hosp, Wuhan 430022, Peoples R China
[3] Nankai Univ, TEDA Coll, TEDA Sch Biol Sci & Biotechnol, Tianjin 300457, Peoples R China
[4] Nankai Univ, TEDA Coll, Tianjing State Lab Microbial Funct Genom, Tianjin 300457, Peoples R China
[5] Univ New S Wales, Sch Biotechnol & Biomol Sci, Sydney, NSW 2052, Australia
[6] TEDA, Tiajin Biochip Technol Corp, Tianjin 300457, Peoples R China
[7] Univ Sydney, Dept Med, Sydney, NSW 2066, Australia
[8] Childrens Hosp, Natl Ctr Immunisat Res & Surveillance Vaccine Pre, Westmead, NSW 2145, Australia
关键词
D O I
10.1099/jmm.0.45924-0
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
In a previous study, a molecular capsular type (MCT) prediction system for 51 Streptococcus pneumoniae serotypes was developed based on a combination of partial cpsA-cpsB sequencing and serotype(s)/group(s)-specific PCR. In this study, another 169 S. pneumoniae isolates were added to the existing database of 427 isolates, including representatives of all 39 serotypes not previously studied. In addition to the authors' own limited sequence data for ail 90 serotypes, cpsA-cpsB sequence data published by the S. pneumoniae capsular loci-sequencing group (http:// www.sanger.ac.uk/Projects/S_pneumoniae/CPS/) at the Sanger Institute or available from GenBank were incorporated into the database. All serotypes, except 25A, were represented by at least two isolates. The number of sequence types identified was 138, of which 110 corresponded to single conventional serotypes (CSs); of these, 57 were represented by two or more isolates. Twenty-six sequence types were shared by between two and four CSs. To resolve these shared cpsA-cpsB sequence types and increase the discriminatory power of our system, the genes encoding the capsular polysaccharide flippase (wzx) and polymerase (wzy) were annotated and 24 new serotype(s)/group(s)-specific PCRs targeting wzy and two targeting wzx were designed. Using both cpsA-cpsB sequencing and wzx/wzy PCR, MCT correctly predicted the CSs of 516 (73 %) and the serogroup of an additional 155 (22 %) of the 708 isolates evaluated. For 5 % of isolates, MCT could not distinguish between members of five serotype pairs (37 isolates) containing members of different serogroups. Although further study of the relationship between MCT and CS is needed, this system now allows serotype or serogroup identification of 95 % of S. pneumoniae isolates.
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收藏
页码:351 / 356
页数:6
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