Synthesis of a fluorescent 7-methylguanosine analog and a fluorescence spectroscopic study of its reaction with wheatgerm cap binding proteins

被引：28

作者：

Ren, JH ^{[1
]}

Goss, DJ ^{[1
]}

机构：

[1] CUNY HUNTER COLL,DEPT CHEM,NEW YORK,NY 10021

来源：

NUCLEIC ACIDS RESEARCH | 1996年 / 24卷 / 18期

基金：

美国国家科学基金会;

关键词：

D O I：

10.1093/nar/24.18.3629

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In the initiation of protein synthesis, the mRNA 5'-terminal 7-methylguanosine cap structure and several recognition proteins play a pivotal role. For the study of this cap binding reaction, one approach is to use fluorescence spectroscopy. A ribose diol-modified fluorescent cap analog, anthraniloyl-m(7)GTP (Antm(7)GTP), was designed and synthesized for this purpose. This fluorescent cap analog was found to have a high quantum yield, resistance to photobleaching and avoided overlap of excitation and emission wavelengths with those of proteins, The binding of Ant-m(7)GTP with wheatgerm initiation factors eIF-4F and eIF-(iso)4F was determined. The fluorescent cap analog and m(7)GTP had similar interactions with both cap binding proteins. Fluorescence quenching experiments showed that the microenvironment of Ant-m(7)GTP when bound to protein was hydrophobic.

引用

页码：3629 / 3634

页数：6

共 37 条

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