Family-10 and family-11 xylanases differ in their capacity to enhance the bleachability of hardwood and softwood paper pulps

被引:54
作者
Clarke, JH
Rixon, JE
Ciruela, A
Gilbert, HJ
Hazlewood, GP
机构
[1] BABRAHAM INST, DEPT CELLULAR PHYSIOL, CAMBRIDGE CB2 4AT, ENGLAND
[2] UNIV NEWCASTLE UPON TYNE, DEPT BIOL & NUTR SCI, NEWCASTLE UPON TYNE NE1 7RU, TYNE & WEAR, ENGLAND
基金
英国生物技术与生命科学研究理事会;
关键词
D O I
10.1007/s002530051035
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Enzyme-aided bleaching of softwood and hardwood kraft pulps by glycosyl hydrolase family-10 and -11 xylanases and a family-26 mannanase was investigated. The ability to release reducing sugar from pulp xylan and to enhance bleachability is not a characteristic shared by all xylanases. Of the six enzymes tested, two xylanases belonging to family 11 were most effective at increasing bleachability and improving final paper brightness. None of the enzymes had a deleterious effect on pulp fibre integrity. The efficiency of individual xylanases as bleach enhancers was not dependent on the source microorganism, and could not be predicted solely on the basis of the quantity or nature of products released from pulp xylan. Cooperative interactions between xylanase/xylanase and xylanase/mannanase combinations, during the pretreatment of softwood and hardwood pulps, were investigated. Synergistic effects on reducing-sugar release and kappa number reduction were elicited by a combination of two family-10 xylanases. Pretreatment of kraft pulp with mannanase A from Pseudomonas flourescens subsp. cellulosa and any one of a number of xylanases resulted in increased release of reducing sugar and a larger reduction in kappa number than obtained with the xylanases alone, confirming the beneficial effects of family-26 mannanases on enzyme-aided bleaching of paper pulp.
引用
收藏
页码:177 / 183
页数:7
相关论文
共 19 条
[1]  
[Anonymous], 1936, 2 REPORT PULP EVALUA
[2]  
[Anonymous], [No title captured]
[3]   A NON-MODULAR ENDO-BETA-1,4-MANNANASE FROM PSEUDOMONAS-FLUORESCENS SUBSPECIES CELLULOSA [J].
BRAITHWAITE, KL ;
BLACK, GW ;
HAZLEWOOD, GP ;
ALI, BRS ;
GILBERT, HJ .
BIOCHEMICAL JOURNAL, 1995, 305 :1005-1010
[4]  
CLARK TA, 1991, APPITA J, V44, P389
[5]  
Clarke JH, 1996, FEMS MICROBIOL LETT, V139, P27, DOI 10.1016/0378-1097(96)00101-2
[6]   DIFFERENTIAL AND SYNERGISTIC ACTION OF STREPTOMYCES ENDOXYLANASES IN PREBLEACHING OF KRAFT PULPS [J].
ELEGIR, G ;
SYKES, M ;
JEFFRIES, TW .
ENZYME AND MICROBIAL TECHNOLOGY, 1995, 17 (10) :954-959
[7]   CLONING OF A THERMOMONOSPORA-FUSCA XYLANASE GENE AND ITS EXPRESSION IN ESCHERICHIA-COLI AND STREPTOMYCES-LIVIDANS [J].
GHANGAS, GS ;
HU, YJ ;
WILSON, DB .
JOURNAL OF BACTERIOLOGY, 1989, 171 (06) :2963-2969
[8]   HOMOLOGOUS CATALYTIC DOMAINS IN A RUMEN FUNGAL XYLANASE - EVIDENCE FOR GENE DUPLICATION AND PROKARYOTIC ORIGIN [J].
GILBERT, HJ ;
HAZLEWOOD, GP ;
LAURIE, JI ;
ORPIN, CG ;
XUE, GP .
MOLECULAR MICROBIOLOGY, 1992, 6 (15) :2065-2072
[9]   BACTERIAL CELLULASES AND XYLANASES [J].
GILBERT, HJ ;
HAZLEWOOD, GP .
JOURNAL OF GENERAL MICROBIOLOGY, 1993, 139 :187-194
[10]   DOMAINS IN MICROBIAL BETA-1,4-GLYCANASES - SEQUENCE CONSERVATION, FUNCTION, AND ENZYME FAMILIES [J].
GILKES, NR ;
HENRISSAT, B ;
KILBURN, DG ;
MILLER, RC ;
WARREN, RAJ .
MICROBIOLOGICAL REVIEWS, 1991, 55 (02) :303-315