Identification of ethylene-mediated protein changes during nodulation in Medicago truncatula using proteome analysis

被引:33
作者
Prayitno, Joko
Imin, Nijat
Rolfe, Barry G.
Mathesius, Ulrike [1 ]
机构
[1] Australian Natl Univ, Sch Biochem & Mol Sci, ARC Ctr Excellence Integrat Legume Res, Canberra, ACT 0200, Australia
[2] Australian Natl Univ, Res Sch Biol Sci, ARC Ctr Excellence Integrat Legume Res, Genom Interact Grp, Canberra, ACT 0200, Australia
关键词
ethylene; Medicago truncatula; model legume; nodulation; protein identification; sickle mutant; Sinorhizobium meliloti;
D O I
10.1021/pr0602646
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Ethylene has been hypothesised to be a regulator of root nodule development in legumes, but its molecular mechanisms of action remain unclear. The skl mutant is an ethylene-insensitive legume mutant showing a hypernodulation phenotype when inoculated with its symbiont Sinorhizobium meliloti. We used the skl mutant to study the ethylene-mediated protein changes during nodule development in Medicago truncatula. We compared the root proteome of the skl mutant to its wildtype in response to the ethylene precursor aminocyclopropane carboxylic acid (ACC) to study ethylene-mediated protein expression in root tissues. We then compared the proteome of skl roots to its wildtype after Sinorhizobium inoculation to identify differentially displayed proteins during nodule development at 1 and 3 days post inoculation (dpi). Six proteins (pprg-2, Kunitz proteinase inhibitor, and ACC oxidase isoforms) were down-regulated in skl roots, while three protein spots were up-regulated (trypsin inhibitor, albumin 2, and CPRD49). ACC induced stress-related proteins in wildtype roots, such as pprg-2, ACC oxidase, proteinase inhibitor, ascorbate peroxidase, and heat-shock proteins. However, the expression of stress-related proteins such as pprg-2, Kunitz proteinase inhibitor, and ACC oxidase, was down-regulated in inoculated skl roots. We hypothesize that during early nodule development, the plant induces ethylene-mediated stress responses to limit nodule numbers. When a mutant defective in ethylene signaling, such as skl, is inoculated with rhizobia, the plant stress response is reduced, resulting in increased nodule numbers.
引用
收藏
页码:3084 / 3095
页数:12
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