Hormone treatment enhances WT1 activation of Renilla luciferase constructs in LNCaP cells

The zinc finger transcription factor, WT1, regulates many growth control genes, repressing or activating transcription depending on the gene and cell type. Based on earlier analyses of the effect of WT1 on androgen responsive genes, we hypothesized that there may be an interaction between the androgen signaling pathway and WT1, such that the commonly used Renilla luciferase control vectors were activated in LNCaP prostate cancer cells. Using co- transfection assays we tested the effects of WT1 and/ or the androgen analog, R1881, on two Renilla luciferase vectors, pRL- SV40 and the promoter- less pRL-null. To determine whether the zinc finger DNA binding domain was required, the zinc finger mutant DDS- WT1 ( R394W) was tested; but it had no significant effect on the Renilla luciferase vectors. To determine whether the androgen signaling pathway was required, WT1 was cotransfected with Renilla vectors in cells with varied hormone responsiveness. The WT1 effect on pRL- null varied from no significant effect in 293 and PC3 cells to very strong enhancement in LNCaP cells treated with 5nM R1881. Overall, these results suggest that hormone enhanced WT1 mediated activation of Renilla luciferase and that these interactions require an intact WT1 zinc finger DNA binding domain.