Preparation of adsorbents applicable to pseudobiospecific ligand affinity chromatography using different spacers and ligands

被引：24

作者：

Alvarez, CI ^{[1
]}

Strumia, MC ^{[1
]}

Bertorello, HE ^{[1
]}

机构：

[1] NATL UNIV CORDOBA,FAC CIENCIAS QUIM,DEPT QUIM ORGAN,RA-5016 CORDOBA,ARGENTINA

来源：

REACTIVE & FUNCTIONAL POLYMERS | 1997年 / 34卷 / 01期

关键词：

epoxy-activated matrix; pseudobiospecific ligand affinity chromatography; histidine ligand; immobilized metal ligand; dye ligand;

D O I：

10.1016/S1381-5148(97)00026-6

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

In this paper, a series of different adsorbents for pseudobiospecific ligand affinity chromatography is presented. They were obtained using ligands as histidine, Cu2+- or Zn2+-chelating iminodiacetic acid and Cibacron Blue F3GA dye, attached to poly(butadiene-hydroxyethylmethacrylate) (PB-HEMA) matrix. The histidine and metal-ion-chelating iminodiacetic acid were attached on matrix through epichlorohydrin (ECH) or 1,4-butanediol diglycidyl ether (EDGE) as spacers and activators, while dye was directly bound onto the matrix.

引用

页码：103 / 111

页数：9

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