Role of protein kinase C and carboxyl-terminal region in acute desensitization of vasopressin V1a receptor

被引：12

作者：

Ancellin, N

Preisser, L

Corman, B

Morel, A

机构：

[1] CTR ETUD SACLAY, CEA, SERV BIOL CELLULAIRE, F-91191 GIF SUR YVETTE, FRANCE

[2] UNIV ANGERS, F-49000 ANGERS, FRANCE

来源：

FEBS LETTERS | 1997年 / 413卷 / 02期

关键词：

vasopressin V1a receptor; protein kinase C; desensitization; intracellular calcium; Xenopus oocyte;

D O I：

10.1016/S0014-5793(97)00914-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The role of protein kinase C activation and carboxyl-terminal region in rapid desensitization of the vasopressin Via receptor was investigated in Xenopus oocytes. Preincubation of the oocytes with vasopressin or with the diacylglycerol analog 1-oleoyl-2-acetyl-sn-glycerol (GAG), or direct injection of active protein kinase C, all blunted the calcium response of the Via receptor, Truncation of the 51 terminal amino acids (S374STOP) modified neither the intracellular calcium response to vasopressin nor its desensitization by vasopressin or GAG. These data suggest that desensitization of the Via receptor is mediated by PKC activation and that its carboxyl-terminal domain is not required for signal transduction and rapid desensitization, (C) 1997 Federation of European Biochemical Societies.

引用

页码：323 / 326

页数：4

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