Immunoprotective activity and safety of a respiratory syncytial virus vaccine: Mucosal delivery of fusion glycoprotein with a CpG oligodeoxynucleotide adjuvant

被引：46

作者：

Prince, GA

Mond, JJ

Porter, DD

Yim, KC

Lan, SJ

Klinman, DM

机构：

[1] Vir Syst Inc, Rockville, MD 20850 USA

[2] US FDA, Ctr Biol Evaluat & Res, Sect Retroviral Immunol, Bethesda, MD 20892 USA

[3] Univ Calif Los Angeles, Sch Med, Dept Pathol & Lab Med, Los Angeles, CA 90095 USA

[4] Biosynexus Inc, Gaithersburg, MD 20877 USA

来源：

JOURNAL OF VIROLOGY | 2003年 / 77卷 / 24期

关键词：

D O I：

10.1128/JVI.77.24.13156-13160.2003

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

CpG oligodeoxynucleotides (ODN) were identified that stimulated immunoglobulin production and cell proliferation in cotton rat cells in vitro. Three of these ODN were used as a mucosall adjuvant in the noses of cotton rats immunized via this route with respiratory syncytial virus fusion (F) protein. The CpG ODN markedly increased the cotton rat humoral neutralizing-antibody response to respiratory syncytial virus. Such immunized animals had a marked reduction in the production of infectious virus after a live-virus challenge. Animals immunized with the combination of F protein and CpG developed enhanced pulmonary pathology consisting of alveolitis and interstitial pneumonitis after a live-virus challenge. Similar enhanced disease has been seen in cotton rats and children immunized with formalin-inactivated respiratory syncytial virus.

引用

页码：13156 / 13160

页数：5

共 29 条

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