Amplified fragment length polymorphism (AFLP) analysis of Clostridium perfringens for epidemiological typing

被引：61

作者：

McLauchlin, J ^{[1
]}

Ripabelli, G ^{[1
]}

Brett, MM ^{[1
]}

Threlfall, EJ ^{[1
]}

机构：

[1] Cent Publ Hlth Lab, Publ Hlth Lab Serv, Div Gastrointestinal Infect, London NW9 5HT, England

来源：

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY | 2000年 / 56卷 / 01期

关键词：

AFLP; Clostridium perfringens; epidemiological typing;

D O I：

10.1016/S0168-1605(00)00227-0

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Thirty-five Clostridium pelfringens isolates from patients and foods implicated in seven outbreaks of suspected CI. perfringens food poisoning together with five unrelated incidents were analysed by serotyping and amplified fragment length polymorphism (AFLP). Despite minor band differences, AFLP was found to be highly reproducible and 16 different profiles (each unique to the 12 incidents) were recognised. The results from both serotyping and AFLP analysis identified exactly the same groups of related cultures. It is concluded that AFLP can provide a rapid, sensitive and reproducible method for the typing of CE. perfringens for outbreak investigation. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：21 / 28

页数：8

共 23 条

[1]

BERRY PR, 1991, ANAEROBES HUMAN DISE, P362

[2] RAPID AND SIMPLE METHOD FOR PURIFICATION OF NUCLEIC-ACIDS [J].

BOOM, R ;

SOL, CJA ;

SALIMANS, MMM ;

JANSEN, CL ;

WERTHEIMVANDILLEN, PME ;

VANDERNOORDAA, J .

JOURNAL OF CLINICAL MICROBIOLOGY, 1990, 28 (03) :495-503

[3] AFLP allows the identification of genomic markers of ruminant Chlamydia psittaci strains useful for typing and epidemiological studies [J].

Boumedine, KS ;

Rodolakis, A .

RESEARCH IN MICROBIOLOGY, 1998, 149 (10) :735-744

[4]

Duim B, 1999, APPL ENVIRON MICROB, V65, P2369

[5] RIBOTYPING OF CLOSTRIDIUM-PERFRINGENS ISOLATES [J].

FORSBLOM, B ;

PALMU, A ;

HIRVONEN, P ;

JOUSIMIESSOMER, H .

CLINICAL INFECTIOUS DISEASES, 1995, 20 :S323-S324

[6]

Geornaras I, 1999, APPL ENVIRON MICROB, V65, P3828

[7] Use of an amplified-fragment length polymorphism technique to fingerprint and differentiate isolates of Helicobacter pylori [J].

Gibson, JR ;

Slater, E ;

Xerry, J ;

Tompkins, DS ;

Owen, RJ .

JOURNAL OF CLINICAL MICROBIOLOGY, 1998, 36 (09) :2580-2585

[8]

Hatheway CL, 1998, Topley and Wilson's microbiology and microbial infection, V2, P731

[9] Evaluation of the DNA fingerprinting method AFLP as a new tool in bacterial taxonomy [J].

Janssen, P ;

Coopman, R ;

Huys, G ;

Swings, J ;

Bleeker, M ;

Vos, P ;

Zabeau, M ;

Kersters, K .

MICROBIOLOGY-UK, 1996, 142 :1881-1893

[10] Molecular evolution and diversity in Bacillus anthracis as detected by amplified fragment length polymorphism markers [J].

Keim, P ;

Kalif, A ;

Schupp, J ;

Hill, K ;

Travis, SE ;

Richmond, K ;

Adair, DM ;

HughJones, M ;

Kuske, CR ;

Jackson, P .

JOURNAL OF BACTERIOLOGY, 1997, 179 (03) :818-824

← 1 2 3 →