Selection and characterization of human antibodies against hepatitis B virus surface antigen (HBsAg) by phage-display

被引：19

作者：

Kim, SH ^{[1
]}

Park, SY ^{[1
]}

机构：

[1] Korea Green Cross Corp, Antibody Engn Lab, Cent Res Ctr, Yongin 449903, Kyunggi Do, South Korea

来源：

HYBRIDOMA AND HYBRIDOMICS | 2002年 / 21卷 / 05期

关键词：

D O I：

10.1089/153685902761022742

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Hepatitis B virus (HBV) is one of the main pathogens of hepatitis and hepatocarcinoma. Human plasma-derived antibody to HBV is being used as a prophylactic for postexposure to HBV and liver transplantation currently. However, it is required to replace the plasma-derived anti-HBs antibody (Ab) to a recombinant antibody because of limited availability of human plasma with high anti-HBs Ab titer and possible contamination of human pathogens. We constructed an anti-HBs Ab-enriched phage-display library from peripheral blood B cells of vaccinated volunteers and the size of library was similar to1.0 x 10(7). The library was panned against hepatitis B surface antigen (HBsAg) and five different clones were isolated. All five clones exhibited the same heavy chain sequence; in contrast, light-chain exhibited one lambda and four different kappa sequences. The Fabs were expressed soluble in E. coli and exhibited affinities of 2.1 x 10(8) similar to 7.7 x 10(8) M-1.

引用

页码：385 / 392

页数：8

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