Increased in vitro interleukin-12 production by peripheral blood in high-risk IDDM first degree relatives

被引:25
作者
Szelachowska, M
Kretowski, A
Kinalska, I
机构
[1] Department of Endocrinology, Medical School Bialystok, Bialystok
[2] Department of Endocrinology, Medical School Bialystok, P-15-276 Bialystok
关键词
type 1 diabetes mellitus; interleukin-12; pathogenesis;
D O I
10.1055/s-2007-979014
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Cytokines secreted by antigen presenting cells, lymphocytes T and pancreatic beta cells are considered as the major mediators in the pathogenesis of IDDM. It has been suggested that cytokines released by macrophages/monocytes could have an initial role in beta-cell damage. The aim of the present study was the estimation of in vitro production of macrophage-derived cytokines: IL-1 beta, TNF-alpha, IL-12 by peripheral blood in high risk IDDM first degree relatives, since it could reflect early events leading to the development of type 1 diabetes in humans. The study was performed in 25 high risk IDDM subjects and 21 age and sex-matched healthy controls. IL-1 beta, TNF-alpha and IL-12 concentrations in supernatants of whole blood cultures with PHA (10 mu g/ml) were quantified by ELISA. In the ICA positive relatives of IDDM subjects levels of IL-12 were significantly higher as compared with the control group, both at 48 h (p < 0.02) and at 72 h (p < 0.05) of incubation and positively correlated with TNF-alpha and IL-1 beta (R = 0.46, p < 0.02 and R = 0.32, p < 0.05). We did not observe statistical differences in in vitro production of TNF-alpha and IL-1 beta between the study groups. In conclusion we suggest that our findings support the hypothesis, that IL-12 is involved in the pathogenesis of human IDDM. If the involvement of Th1 cells is confirmed in the destruction of islet beta-cells, it is possible that IL-12 antagonists will have a role in the future prevention of insulin dependent diabetes mellitus.
引用
收藏
页码:168 / 171
页数:4
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