Structural basis of transcription: Role of the trigger loop in substrate specificity and catalysis

被引：369

作者：

Wang, Dong ^{[1
]}

Bushnell, David A. ^{[1
]}

Westover, Kenneth D. ^{[1
]}

Kaplan, Craig D. ^{[1
]}

Kornberg, Roger D. ^{[1
]}

机构：

[1] Stanford Univ, Sch Med, Dept Biol Struct, Stanford, CA 94305 USA

来源：

CELL | 2006年 / 127卷 / 05期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/j.cell.2006.11.023

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

New structures of RNA polymerase II (pol II) transcribing complexes reveal a likely key to transcription. The trigger loop swings beneath a correct nucleoside triphosphate (NTP) in the nucleotide addition site, closing off the active center and forming an extensive network of interactions with the NTP base, sugar, phosphates, and additional pol II residues. A histidine side chain in the trigger loop, precisely positioned by these interactions, may literally "trigger" phosphodiester bond formation. Recognition and catalysis are thus coupled, ensuring the fidelity of transcription.

引用

页码：941 / 954

页数：14

共 59 条

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