Cell-mediated drug delivery by gingival interdental papilla mesenchymal stromal cells (GinPa-MSCs) loaded with paclitaxel

被引：53

作者：

Brini, Anna Teresa ^{[1
,2
]}

Cocce, Valentina ^{[1
,4
]}

Ferreira, Lorena M. Jose ^{[1
]}

Giannasi, Chiara ^{[1
]}

Cossellu, Gianguido ^{[3
]}

Gianni, Aldo Bruno ^{[4
]}

Angiero, Francesca ^{[5
]}

Bonomi, Arianna ^{[1
]}

Pascucci, Luisa ^{[6
]}

Falchetti, Maria Laura ^{[7
]}

Ciusani, Emilio ^{[8
]}

Bondiolotti, Gianpietro ^{[9
]}

Sisto, Francesca ^{[1
]}

Alessandri, Giulio ^{[10
]}

Pessina, Augusto ^{[1
]}

Farronato, Giampietro ^{[1
,3
]}

机构：

[1] Univ Milan, Dept Biomed Surg & Dent Sci, Via Pascal 36, I-20133 Milan, Italy

[2] IRCCS Ist Ortoped Galeazzi, Milan, Italy

[3] Univ Milan, Dept Biomed Surg & Dent Sci, Fdn IRCCS Ca Granda Osped Maggiore Policlin, Unit Orthodont & Paediat Dent,Sch Dent, Milan, Italy

[4] Univ Milan, Dept Biomed Surg & Dent Sci, Fdn IRCCS Ca Granda Osped Maggiore Policlin, Maxillofacial & Dent Unit, Milan, Italy

[5] Univ Genoa, S Martino Hosp, Dept Med Sci & Diagnost Integrated, Genoa, Italy

[6] Univ Perugia, Dept Vet Med, I-06100 Perugia, Italy

[7] CNR, Inst Cell Biol & Neurobiol, Rome, Italy

[8] Fdn IRCCS Neurol Inst Carlo Besta, Lab Clin Pathol & Neurogenet Med, Milan, Italy

[9] Univ Milan, Dept Med Biotechnol & Translat Med, Milan, Italy

[10] IRCCS Neurol Inst C Besta, Dept Cerebrovasc Dis, Cellular Neurobiol Lab, Milan, Italy

来源：

EXPERT OPINION ON DRUG DELIVERY | 2016年 / 13卷 / 06期

关键词：

CFPAC-1; drug delivery; gingival mesenchymal stromal cells; paclitaxel; STEM-CELLS; IN-VITRO; PANCREATIC ADENOCARCINOMA; PERIODONTAL-LIGAMENT; DIFFERENTIATION; FIBROBLASTS; REGENERATION; VEHICLES; BIOLOGY;

D O I：

10.1517/17425247.2016.1167037

中图分类号：

R9 [药学];

学科分类号：

100702 [药剂学];

摘要：

Objective: Gingival tissue is composed of cell types that contribute to the body's defense against many agents in oral environment, wound healing and tissue regeneration. Thanks to their easy and scarcely invasive withdrawal procedure, interdental papilla provide a good source of mesenchymal stromal cells (GinPa-MSCs). We isolated GinPa-MSCs and verified their ability to uptake/release the anticancer agent Paclitaxel (PTX). Methods: In vitro expanded GinPa-MSCs were characterized for CD markers by FACS, tested for differentiation ability and analyzed by TEM. Their ability to uptake/release PTX was assessed according to a standardized procedure. Results: The CD expression and chondro-adipo-osteo differentiation ability confirmed the mesenchymal feature of GinPa-MSCs. Surprisingly, 28% of GinPa-MSCs expressed CD14 marker and had an impressive pinocytotic activity. GinPa-MSCs were able to take up and release a sufficient amount of PTX to demonstrate effective in vitro activity against pancreatic carcinoma cells, suggesting that the drug was not inactivated. Conclusions: The procedure to obtain MSCs from interdental papilla is less invasive than that used for both bone marrow and adipose tissue, GinPa-MSCs are easy to expand and can be efficiently loaded with PTX. Taken together these qualities suggest that GinPa-MSCs may prove to be a good tool for cell-mediated drug delivery in cancer, particularly if related to stomatognathic system.

引用

页码：789 / 798

页数：10

共 40 条

[1]

Tissue-derived mesenchymal stromal cells used as vehicles for anti-tumor therapy exert different in vivo effects on migration capacity and tumor growth [J].