Cryopreservation of porcine articular cartilage: MRI and biochemical results after different freezing protocols

被引:29
作者
Laouar, Leila
Fishbein, Ken
McGann, Locksley E.
Horton, Walter E.
Spencer, Richard G.
Jomha, Nadr M.
机构
[1] NIA, Nucl Magnet Resonance Unit, NIA, Intramural Res Program, Baltimore, MD 21224 USA
[2] Univ Alberta Hosp, Dept Surg, Edmonton, AB T6G 2B7, Canada
[3] Northeastern Ohio Univ Coll Med & Pharm, Dept Anat, Rootstown, OH 44272 USA
关键词
articular cartilage; cryopreservation; MRI; dimethyl sulfoxide; biochemical; porcine;
D O I
10.1016/j.cryobiol.2006.10.193
中图分类号
Q [生物科学];
学科分类号
07 [理学]; 0710 [生物学]; 09 [农学];
摘要
The objective of this study was to investigate the effects of cryopreservation on the components of articular cartilage (AC) matrix by utilizing magnetic resonance imaging (MRI) and biochemical assessments. Porcine AC (10 mm osteochondral dowels) was collected into four groups - (1) phosphate buffered saline (PBS) control, (2) PBS snap frozen in liquid nitrogen, (3) slow-cooled in dimethyl sulfoxide (DMSO), and (4) slow cooled in PBS (in absence of DMSO). MRI results demonstrated three distinct zones in the cartilage. After exposure to ice formation during cryopreservation procedures, alterations in MRI determined matrix fixed charged density and magnetization transfer rate were noted. In addition, biochemical assays demonstrated significant alterations in chondroitin sulfate and hydroxyproline content over time without differences in hydration or DNA content. In conclusion, MRI was able to detect some changes in the intact cartilage matrix structure consistent with biochemical assessments after ice formation during cryopreservation of intact porcine AC. Furthermore, biochemical assessments supported some of these findings and changed significantly after incubating the cartilage matrix for 36-72 h in PBS in terms of chondroitin sulfate and hydroxyproline content. (c) 2006 Elsevier Inc. All rights reserved.
引用
收藏
页码:36 / 43
页数:8
相关论文
共 38 条
[1]
Analysis of stored osteochondral allografts at the time of surgical implantation [J].
Allen, RT ;
Robertson, CM ;
Pennock, AT ;
Bugbee, WD ;
Harwood, FL ;
Wong, VW ;
Chen, AC ;
Sah, RL ;
Amiel, D .
AMERICAN JOURNAL OF SPORTS MEDICINE, 2005, 33 (10) :1479-1484
[2]
Osteochondritis dissecans of the femoral condyles - Long-term results of excision of the fragment [J].
Anderson, AF ;
Pagnani, MJ .
AMERICAN JOURNAL OF SPORTS MEDICINE, 1997, 25 (06) :830-834
[3]
[Anonymous], 1994, ORTHOPAEDIC BASIC SC
[4]
AUBIN PP, 2001, CLIN ORTHOP S, V391, P318, DOI DOI 10.1097/00003086-200110001-00029
[5]
Ball ST, 2004, CLIN ORTHOP RELAT R, P246
[6]
Glycosaminoglycan in articular cartilage: In vivo assessment with delayed Gd(DTPA)(2-)-enhanced MR imaging [J].
Bashir, A ;
Gray, ML ;
Boutin, RD ;
Burstein, D .
RADIOLOGY, 1997, 205 (02) :551-558
[7]
Gd-DTPA(2-) as a measure of cartilage degradation [J].
Bashir, A ;
Gray, ML ;
Burstein, D .
MAGNETIC RESONANCE IN MEDICINE, 1996, 36 (05) :665-673
[8]
Osteochondral allograft transplantation [J].
Bugbee, WD ;
Convery, FR .
CLINICS IN SPORTS MEDICINE, 1999, 18 (01) :67-+
[9]
OSTEOARTICULAR ALLOGRAFTS FOR RECONSTRUCTION AFTER RESECTION OF A MUSCULOSKELETAL TUMOR IN THE PROXIMAL END OF THE TIBIA [J].
CLOHISY, DR ;
MANKIN, HJ .
JOURNAL OF BONE AND JOINT SURGERY-AMERICAN VOLUME, 1994, 76A (04) :549-554
[10]
CONVERY FR, 1991, CLIN ORTHOP RELAT R, V273, P139