Simultaneous quantitative determination of the major phase I and II metabolites of ibuprofen in biological fluids by high-performance liquid chromatography on dynamically modified silica

被引:29
作者
Kepp, DR [1 ]
Sidelmann, UG [1 ]
Tjornelund, J [1 ]
Hansen, SH [1 ]
机构
[1] ROYAL DANISH SCH PHARM, DEPT ANALYT & PHARMACEUT CHEM, DK-2100 COPENHAGEN, DENMARK
来源
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 1997年 / 696卷 / 02期
关键词
ibuprofen;
D O I
10.1016/S0378-4347(97)00239-9
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Ibuprofen has previously, after ingestion by man, been demonstrated to yield four major phase I metabolites, which are excreted in the urine partly as glucuronic acid conjugates. However, in previous investigations the quantitative determinations of the conjugates were performed by indirect methods. The purpose of the present investigation was to develop a high-performance liquid chromatographic (HPLC) system for the simultaneous determination of the major phase I and II metabolites of ibuprofen in biological fluids. The separation was performed using bare silica dynamically modified with N-cetyl-N,N,N-trimethylammonium hydroxide ions contained in the mobile phase. The separation of the metabolites of ibuprofen is greatly improved with this system compared to other published reversed-phase HPLC systems intended for the same purpose. The method developed makes it possible to simultaneously determine the intact glucuronic acid conjugates of ibuprofen as well as its phase I metabolites in human urine. In a study involving four healthy volunteers, a total recovery in urine of the dose given was found to be 58-86% within 8 h. This may be compared to an average of 67% earlier reported in the literature. (C) 1997 Elsevier Science B.V.
引用
收藏
页码:235 / 241
页数:7
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