Estimation of mineral-adhered biomass of Thiobacillus ferooxidans by protein assay - Some problems and remedies

被引：17

作者：

Karan, G

Natarajan, KA

Modak, JM

机构：

[1] INDIAN INST SCI,DEPT MET,BANGALORE 560012,KARNATAKA,INDIA

[2] INDIAN INST SCI,DEPT CHEM ENGN,BANGALORE 560012,KARNATAKA,INDIA

来源：

HYDROMETALLURGY | 1996年 / 42卷 / 02期

关键词：

D O I：

10.1016/0304-386X(95)00080-Z

中图分类号：

TF [冶金工业];

学科分类号：

0806 ;

摘要：

Enumeration of adhered cells of Thiobacillus ferrooxidans on sulphide minerals through protein assay poses problems due to interference from dissolved mineral constituents. The manner in which sulphide minerals such as pyrite, chalcopyrite, sphalerite, arsenopyrite and pyrrhotite interfere with bacterial protein estimation is demonstrated. Such interferences can be minimised either through dilution or addition of H2O2 to the filtrate after hot alkaline digestion of the biotreated mineral samples.

引用

页码：169 / 175

页数：7

共 10 条

[1]

[Anonymous], 1988, BIOGEOTECHNOLOGY MET, DOI DOI 10.1007/s10533-005-3655-z

[2] DETECTION OF THIOBACILLUS-FERROOXIDANS IN ACID MINE ENVIRONMENTS BY INDIRECT FLUORESCENT-ANTIBODY STAINING [J].