Rapid amperometric detection of coliforms based on MWNTs/Nafion composite film modified glass carbon electrode

被引：37

作者：

Cheng, Yuxiao ^{[1
]}

Liu, Yajun ^{[1
]}

Huang, Jingjing ^{[1
]}

Man, Yuezhong ^{[1
]}

Zhang, Wen ^{[1
]}

Zhang, Zhonghai ^{[1
]}

Jin, Litong ^{[1
]}

机构：

[1] E China Normal Univ, Dept Chem, Shanghai 200062, Peoples R China

来源：

TALANTA | 2008年 / 75卷 / 01期

基金：

中国国家自然科学基金;

关键词：

multi-wall carbon nanotubes; nafion; coliforms; amperometric detection; beta-galactosidase; p-aminophenol;

D O I：

10.1016/j.talanta.2007.10.047

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A multi-wall carbon nanotubes (MWNTs)/Nafion modified glassy carbon electrode (GCE) was fabricated for the rapid amperometric detection of coliforms, represented by Escherichia coli (E. coli). In the bacterial solution, P-galactosidase which was used as an indicator of coliforms reacted with substrate, p-aminophenol-beta-galactopyranoside (PAPG), and produced p-aminophenol (PAP). PAP was detected by MWNTs/Nafion modified GCE. Due to the cation-exchange capacity of Nation and the electrocatalytic ability of MWNTs, the detection sensitivity of PAP was improved and the detection time of coliforms was shortened. The bacterial can be detected within 5 h ranging from 10 to 10(4) cfu/mL. The MWNTs/Nafion modified GCE was easy to be constructed and regenerated. To our best knowledge, it was the first time to use MWNTs/Nafion modified GCE to detect the concentration of coliforms. (C) 2007 Published by Elsevier B.V.

引用

页码：167 / 171

页数：5

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