Diacylglycerol induces fusion of nuclear envelope membrane precursor vesicles

被引:36
作者
Barona, T
Byrne, RD
Pettitt, TR
Wakelam, MJO
Larijani, B
Poccia, DL [1 ]
机构
[1] Amherst Coll, Dept Biol, Amherst, MA 01002 USA
[2] London Res Inst, Cell Biophys Lab, Canc Res UK, London WC2A 3PX, England
[3] Univ Birmingham, CRUK Inst Canc Studies, Birmingham B15 2TT, W Midlands, England
[4] Univ Lusofona, Unidade Invest Biol Desenvolvimento, P-1749024 Lisbon, Portugal
基金
英国惠康基金;
关键词
D O I
10.1074/jbc.M412863200
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Purified membrane vesicles isolated from sea urchin eggs form nuclear envelopes around sperm nuclei following GTP hydrolysis in the presence of cytosol. A low density subfraction of these vesicles (MV1), highly enriched in phosphatidylinositol ( PtdIns), is required for nuclear envelope formation. Membrane fusion of MV1 with a second fraction that contributes most of the nuclear envelope can be initiated without GTP by an exogenous bacterial PtdIns-specific phospholipase C (PI-PLC) which hydrolyzes PtdIns to form diacyl-glycerides and inositol 1-phosphate. This PI-PLC hydrolyzes a subset of sea urchin membrane vesicle PtdIns into diglycerides enriched in long chain, polyunsaturated species as revealed by a novel liquid chromatography-mass spectrometry analysis. Large unilammelar vesicles (LUVs) enriched in PtdIns can substitute for MV1 in PI-PLC induced nuclear envelope formation. Moreover, MV1 prehydrolyzed with PI-PLC and washed to remove inositols leads to spontaneous nuclear envelope formation with MV2 without further PI-PLC treatment. LUVs enriched in diacylglycerol mimic prehydrolyzed MV1. These results indicate that production of membrane-destabilizing diglycerides in membranes enriched in PtdIns may facilitate membrane fusion in a natural membrane system and suggest that MV1, which binds only to two places on the sperm nucleus, may initiate fusion locally.
引用
收藏
页码:41171 / 41177
页数:7
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